Highlights•Cryo-electro microscopy reveals the native conformation of chromatin fiber both
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …

A long strand of DNA is wrapped around the core histone and forms a nucleosome.
Although the nucleosome has long been assumed to be folded into 30-nm chromatin fibres …

Chromatin is a dynamic molecular complex composed of DNA and proteins that package the
DNA in the nucleus of eukaryotic cells. The basic structural unit of chromatin is the …

The predominant view of chromatin structure is that the beaded chain of nucleosomes is
folded into a symmetrical helical fibre. Recently, however, direct evidence from cryoelectron …

Despite over 30years of work, the fundamental structure of eukaryotic chromatin remains
controversial. Here, we review the roots of this controversy in disparities between results …

In eukaryotic cells, genomic DNA is highly packaged into chromatin to fit inside the nucleus.
The accessibility of DNA is dependent on the packing density of chromatin fibers, which …

The potential of electron microscope tomography as a tool for obtaining three-dimensional
(3D) information about large macromolecular assemblies is greatly extended by automation …

The naked DNA inside the nucleus interacts with proteins and RNAs forming a higher order
chromatin structure to spatially and temporally control transcription in eukaryotic cells. The …

The presence and folding pattern of chromatin in eukaryotic cells remain elusive and
controversial. In this study, we prepared ultra-thin sections of Hela cells with three different …

The organization and dynamics of chromatin fiber play crucial roles in regulating DNA
accessibility for gene expression. Here we combine cryoelectron tomography (cryo-ET), sub …