We studied the chromosome periphery in human HeLa and TG cells using cryomethods in
electron microscopy. A contrasted layer of peripheral chromosomal material (PCM) was …

Although the formation of 30-nm chromatin fibers is thought to be the most basic event of
chromatin compaction, it remains controversial because high-resolution imaging of …

A complex structure, visible by electron microscopy, surrounds each chromosome during
mitosis. The organization of this structure is distinct from that of the chromosomes and the …

A newly developed technique allows cytological (light microscope level) chromosome
preparations to be examined at the electron microscopic level. Ultrathin (50 nm) sections of …

In an effort to understand the arrangement of the basic 30 nm chromatin fibre within
metaphase chromosomes, changes in the organization of prematurely condensed …

Chromatin fibers must fold or coil in the process of chromosome condensation. Patterns of
coiling have been demonstrated for reconstituted chromatin, but the actual trajectories of …

Chromatin fibers have been observed and measured in frozen hydrated sections of three
types of cell (chicken erythrocytes and sperm of Patiria miniata and Thyone briareus) …

Cryoelectron microscopy of vitrified sections has become a powerful tool for investigating the
fine structural features of cellular compartments. In the present study, this approach has …

We investigated the nuclear ultrastructure of HeLa cells using cryotechniques. The cells
were cultured as monolayers, quick‐frozen and cryosubstituted in acetone without chemical …

Using an original high-salt extraction protocol, we observed a novel chromosome
substructure, referred to as the peripheral chromosome scaffold. This chromosome domain …