We describe the results of a systematic study, using electron microscopy, of the effects of
ionic strength on the morphology of chromatin and of H1-depleted chromatin. With …

Electron microscopic and biochemical studies demonstrate that the fundamental structure of
chromatin depleted of lysine-rich histones is composed of a flexible chain of spherical …

The basic chromatin fibre (the 10 nm diameter fibre) is a linear repeating array of
nucleosomes, or nucleosome filament. The core of each disk-like nucleosome is a wedge …

Both intact and nuclease-isolated chromatin fibers have been examined at different degrees
of salt-induced compaction, using a variety of preparation techniques. The results suggest …

We have examined the role played by various histones in the organization of the DNA of the
nucleosome, using staphylococcal nuclease as a probe of DNA conformation. When this …

The arginine-rich histone, H3, isolated from avian erythrocytes, can dimerize by forming a
disulfide linkage between the single cysteine sulfhydryl residues at position 110 of the H3 …

Summary Chromatin subunits (“nucleosomes”) which were purified by sucrose gradient
centrifugation of a staphylococcal nuclease digest of chromatic, have been studied. We …

There is considerable current interest in the organisation of nucleosomes in chromatin. A
strong X-ray and neutron semi-meridional diffraction peak at approximately 10 nm had …

Abstract Effects of non-histone components and histone H1 on the morphology of
nucleosomes and chromatin were studied by electron microscopy. Soluble rat liver …

The role of histone H1 in nucleosome heterogeneity and structure has been studied using a
reconstitution procedure. Histone H1 and non-histone proteins are removed selectively from …