Recovery of adult zebrafish hearts for high-throughput applications

R Arnaout, S Reischauer… - Journal of Visualized …, 2014 - pmc.ncbi.nlm.nih.gov
Use of the zebrafish model system for studying development, regeneration, and disease is
expanding toward use of adult hearts for cell dissociation and purification of RNA, DNA, and …

Heart dissection in larval, juvenile and adult zebrafish, Danio rerio

C Singleman, NG Holtzman - Journal of visualized …, 2011 - pmc.ncbi.nlm.nih.gov
Zebrafish have become a beneficial and practical model organism for the study of embryonic
heart development (see recent reviews1-6), however, work examining post-embryonic …

Cardiac Resection Injury in Zebrafish

DZ Sheng, D Zheng, K Kikuchi - Cardiac Regeneration: Methods and …, 2021 - Springer
The zebrafish (Danio rerio) possesses a spectacular capacity for cardiac regeneration.
Zebrafish have been used in cardiac regeneration research for nearly two decades …

Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device

JK Yip, M Harrison, J Villafuerte, GE Fernandez… - Lab on a Chip, 2020 - pubs.rsc.org
Myocardial infarction and heart failure are leading causes of death worldwide, in large part
because adult human myocardium has extremely limited regeneration capacity. Zebrafish …

Large-scale zebrafish embryonic heart dissection for transcriptional analysis

VA Lombardo, C Otten… - Journal of visualized …, 2015 - pmc.ncbi.nlm.nih.gov
The zebrafish embryonic heart is composed of only a few hundred cells, representing only a
small fraction of the entire embryo. Therefore, to prevent the cardiac transcriptome from …

Live imaging of adult zebrafish cardiomyocyte proliferation ex vivo

H Honkoop, PD Nguyen, VEM van der Velden… - …, 2021 - journals.biologists.com
Zebrafish are excellent at regenerating their heart by reinitiating proliferation in pre-existing
cardiomyocytes. Studying how zebrafish achieve this holds great potential in developing …

Heart on a plate: histological and functional assessment of isolated adult zebrafish hearts maintained in culture

S Pieperhoff, KS Wilson, J Baily, K de Mora… - PloS one, 2014 - journals.plos.org
The zebrafish is increasingly used for cardiovascular genetic and functional studies. We
present a novel protocol to maintain and monitor whole isolated beating adult zebrafish …

Purification of hearts from zebrafish embryos

C Geoffrey Burns, CA MacRae - Biotechniques, 2006 - Taylor & Francis
Have a Heart Obtaining pure organ preparation by microdissection from small organisms,
such as the zebrafish, Dario rerio, is itself a sufficiently arduous task. Isolating such organs in …

Functional evaluation of isolated zebrafish hearts

L Hecker, L Khait, SK Sessions, RK Birla - Zebrafish, 2008 - liebertpub.com
Traditional working heart preparations, based on the original Langendorff setup, are widely
used experimental models that have tremendously advanced the cardiovascular field …

Cauterization as a simple method for regeneration studies in the zebrafish heart

PKV Dyck, N Hockaden, EC Nelson, AR Koch… - Journal of …, 2020 - mdpi.com
In the last two decades, the zebrafish has emerged as an important model species for heart
regeneration studies. Various approaches to model loss of cardiac myocytes and myocardial …