Chromosomes condense during mitosis in most eukaryotes. This transformation involves
rearrangements at the nucleosome level and has consequences for transcription. Here, we …

Chromatin organization has an important role in the regulation of eukaryotic systems.
Although recent studies have refined the three-dimensional models of chromatin …

Kinetochores mediate chromosome segregation during cell division. They assemble on
centromeric nucleosomes and capture spindle microtubules. In budding yeast, a kinetochore …

Chromatin is a dynamic molecular complex composed of DNA and proteins that package the
DNA in the nucleus of eukaryotic cells. The basic structural unit of chromatin is the …

Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we
devise the template-, reference-and selection-free (TRSF) cryo-EM pipeline to …

Nuclear processes depend on the organization of chromatin, whose basic units are cylinder-
shaped complexes called nucleosomes. A subset of mammalian nucleosomes in situ (inside …

Mitotic chromosomes are essential structures for the faithful transmission of replicated
genomic DNA into two daughter cells during cell division. A long strand of DNA is wrapped …

The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in
eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have …

Advanced techniques including the chromosome conformation capture (3C) methodology
and its derivatives are complementing microscopy approaches to study genome …

Successful progression through the cell cycle requires spatial and temporal regulation of
gene transcript levels and the number, positions and condensation levels of chromosomes …