Microcarrier-based stem cell bioprocessing: GMP-grade culture challenges and future trends for regenerative medicine

A Ornelas-González… - Critical Reviews in …, 2021 - Taylor & Francis
Recently, stem cell-based therapies have been proposed as an alternative for the treatment
of many diseases. Stem cells (SCs) are well known for their capacity to preserve themselves …

Microcarrier culture systems for stem cell manufacturing

SM Badenes, A Fernandes-Platzgummer… - Stem cell …, 2016 - Elsevier
Stem cells hold a great promise for the generation of new cell therapies for many currently
untreatable diseases, for human disease modeling or drug discovery. Many of these …

Bioprocessing of human mesenchymal stem cells: From planar culture to microcarrier-based bioreactors

AC Tsai, CA Pacak - Bioengineering, 2021 - mdpi.com
Human mesenchymal stem cells (hMSCs) have demonstrated great potential to be used as
therapies for many types of diseases. Due to their immunoprivileged status, allogeneic …

Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors

QA Rafiq, K Coopman, AW Nienow… - Biotechnology …, 2016 - Wiley Online Library
Production of human mesenchymal stem cells for allogeneic cell therapies requires
scalable, cost‐effective manufacturing processes. Microcarriers enable the culture of …

Scalable microcarrier-based manufacturing of mesenchymal stem/stromal cells

AM de Soure, A Fernandes-Platzgummer… - Journal of …, 2016 - Elsevier
Due to their unique features, mesenchymal stem/stromal cells (MSC) have been exploited in
clinical settings as therapeutic candidates for the treatment of a variety of diseases …

Microcarrier choice and bead-to-bead transfer for human mesenchymal stem cells in serum-containing and chemically defined media

J Leber, J Barekzai, M Blumenstock, B Pospisil… - Process …, 2017 - Elsevier
The production of stem cells for clinical applications requires a suitable mass expansion and
harvest process, which is implemented in a microcarrier-based bioreactor. Important …

Challenges and opportunities in downstream separation processes for mesenchymal stromal cells cultured in microcarrier‐based stirred suspension bioreactors

I Mawji, EL Roberts, T Dang… - Biotechnology and …, 2022 - Wiley Online Library
Mesenchymal stromal cells (MSC) are a promising platform for regenerative medicine
applications because of their multilineage differentiation abilities and ease of collection …

Dissolvable gelatin‐based microcarriers generated through droplet microfluidics for expansion and culture of mesenchymal stromal cells

EX Ng, M Wang, SH Neo, CA Tee… - Biotechnology …, 2021 - Wiley Online Library
Microcarriers are synthetic particles used in bioreactor‐based cell manufacturing of
anchorage‐dependent cells to promote proliferation at efficient physical volumes, mainly by …

Large-scale production of stem cells utilizing microcarriers: a biomaterials engineering perspective from academic research to commercialized products

H Tavassoli, SN Alhosseini, A Tay, PPY Chan, SKW Oh… - Biomaterials, 2018 - Elsevier
Human stem cells, including pluripotent, embryonic and mesenchymal, stem cells play
pivotal roles in cell-based therapies. Over the past decades, various methods for expansion …

Large-scale expansion and differentiation of mesenchymal stem cells in microcarrier-based stirred bioreactors

S Sart, SN Agathos - Bioreactors in Stem Cell Biology: Methods and …, 2016 - Springer
Mesenchymal stem cells (MSCs) have emerged as an important tool for tissue engineering,
thanks to their differentiation potential and their broad trophic activities. However, for clinical …