In recent years, it has become evident that [Fe-S] proteins, such as hydrogenase,
nitrogenase and aconitase, require a complex machinery to assemble and insert their …

The nitrogen-fixing organism Azotobacter vinelandii contains at least two systems that
catalyze formation of [Fe-S] clusters. One of these systems is encoded by nif genes, whose …

Iron-sulfur clusters ([Fe-S] clusters) are assembled on molecular scaffolds and subsequently
used for maturation of proteins that require [Fe-S] clusters for their functions. Previous …

The NifS and NifU proteins from Azotobacter vinelandii are required for the full activation of
nitrogenase. NifS is a homodimeric cysteine desulfurase that supplies the inorganic sulfide …

Abstract The NifS and NifU nitrogen fixation-specific gene products are required for the full
activation of both the Fe-protein and MoFe-protein of nitrogenase from Azotobacter …

The NifU protein is a homodimer that is proposed to provide a molecular scaffold for the
assembly of [Fe-S] clusters uniquely destined for the maturation of the nitrogenase catalytic …

The mechanism of [4Fe-4S] cluster assembly on A-type Fe–S cluster assembly proteins, in
general, and the specific role of NifIscA in the maturation of nitrogen fixation proteins are …

NifS and NifU (encoded by nifS and nifU) are generally dedicated to biogenesis of the
nitrogenase Fe–S cluster in diazotrophs. However, nifS and nifU are not found in N2-fixing …

An IscA homologue within the nif regulon of Azotobacter vinelandii, designated NifIscA, was
expressed in Escherichia coli and purified to homogeneity. Purified NifIscA was found to be …

Biological nitrogen fixation is an inherent trait exclusive to a select number of prokaryotes.
Although molybdenum nitrogenase is the dominant catalyst for dinitrogen reduction, some …