Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study

J Vanderstraeten, MJM da Fonseca… - … for biofuels and …, 2022 - Springer
Background Designer cellulosomes are self-assembled chimeric enzyme complexes that
can be used to improve lignocellulosic biomass degradation. They are composed of a …

A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates

Y Vazana, Y Barak, T Unger, Y Peleg… - Biotechnology for …, 2013 - Springer
Background Select cellulolytic bacteria produce multi-enzymatic cellulosome complexes that
bind to the plant cell wall and catalyze its efficient degradation. The multi-modular …

Analyzing designer cellulosomes for their industrial competitiveness

B Lamote, J Vanderstraeten, N De Ridder… - 15th edition of the …, 2024 - biblio.ugent.be
Designer cellulosomes (DCs) are engineered multi-enzyme complexes that hold significant
promise for efficient hydrolysis of lignocellulosic biomass, a crucial step in the biomass-to …

Designer cellulosomes for enhanced hydrolysis of cellulosic substrates

Y Vazana, S Moraïs, Y Barak, R Lamed… - Methods in Enzymology, 2012 - Elsevier
During the past several years, major progress has been accomplished in the production of
“designer cellulosomes,” artificial enzymatic complexes that were demonstrated to efficiently …

Adaptor scaffoldins: an original strategy for extended designer cellulosomes, inspired from nature

J Stern, S Moraïs, R Lamed, EA Bayer - MBio, 2016 - Am Soc Microbiol
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled
incorporation of recombinant dockerin-containing enzymes. The largest designer …

Current challenges in designer cellulosome engineering

B Lamote, MJM da Fonseca, J Vanderstraeten… - Applied Microbiology …, 2023 - Springer
Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising
carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high …

Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis

J Stern, A Kahn, Y Vazana, M Shamshoum, S Morais… - PloS one, 2015 - journals.plos.org
Degradation of cellulose is of major interest in the quest for alternative sources of renewable
energy, for its positive effects on environment and ecology, and for use in advanced …

Carbohydrate depolymerization by intricate cellulosomal systems

S Moraïs, J Stern, L Artzi, CMGA Fontes… - … : Methods and Protocols, 2023 - Springer
Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through
evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components …

Carbohydrate depolymerization by intricate cellulosomal systems

J Stern, L Artzi, S Moraïs, CMGA Fontes… - … Interactions: Methods and …, 2017 - Springer
Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through
evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components …

Mapping the deformability of natural and designed cellulosomes in solution

J Dorival, S Moraïs, A Labourel, B Rozycki… - … for biofuels and …, 2022 - Springer
Background Natural cellulosome multi-enzyme complexes, their components, and
engineered 'designer cellulosomes'(DCs) promise an efficient means of breaking down …