Assessment of mitochondrial polarization status in living cells based on analysis of the spatial heterogeneity of rhodamine 123 fluorescence staining

EC Toescu, A Verkhratsky - Pflügers Archiv, 2000 - Springer
The mitochondrial membrane potential (Ψ mito) is an important parameter not only of
mitochondrial but also of cellular status. Prolonged mitochondrial depolarization is …

Detection of de-and hyperpolarization of mitochondria of cultured astrocytes and neurons by the cationic fluorescent dye rhodamine 123

S Kahlert, G Zündorf, G Reiser - Journal of neuroscience methods, 2008 - Elsevier
The mitochondrial potential is an essential regulator in cellular physiology and detection of
this parameter in living cells is still under discussion. Here we present a protocol which …

Determination of mitochondrial membrane potential and reactive oxygen species in live rat cortical neurons

DC Joshi, JC Bakowska - Journal of visualized experiments …, 2011 - pmc.ncbi.nlm.nih.gov
Mitochondrial membrane potential (ΔΨm) is critical for maintaining the physiological function
of the respiratory chain to generate ATP. A significant loss of ΔΨm renders cells depleted of …

Visualization of mitochondrial membrane potential in mammalian cells

N Esteras, MJW Adjobo-Hermans, AY Abramov… - Methods in cell …, 2020 - Elsevier
Assessment of the mitochondrial membrane potential (Δψ) in living cells, although not trivial,
can be used to estimate mitochondrial bioenergetic state. For this purpose, fluorescent …

Labeling mitochondria with fluorescent dyes for imaging

B Chazotte - Cold Spring Harbor Protocols, 2009 - cshprotocols.cshlp.org
The eukaryotic cell has evolved to compartmentalize its functions and transport various
metabolites among cellular compartments. Therefore, it is important to study cellular …

Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release

ME Christensen, ES Jansen, W Sanchez… - Methods, 2013 - Elsevier
Mitochondria play a pivotal role in life and death of the cell because they produce the
majority of energy required for survival and also regulate the intrinsic pathway to apoptosis …

Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry

FX Sureda, E Escubedo, C Gabriel… - … : The Journal of the …, 1997 - Wiley Online Library
Mitochondrial membrane potential (MMP) in dissociated rat cerebellar neurons was
measured using rhodamine 123 (Rh 123) as fluorescent dye, and flow cytometry. Dye …

Discrimination of depolarized from polarized mitochondria by confocal fluorescence resonance energy transfer

SP Elmore, Y Nishimura, T Qian, B Herman… - Archives of biochemistry …, 2004 - Elsevier
Mitochondrial depolarization promotes apoptotic and necrotic cell death and possibly other
cellular events. Polarized mitochondria take up cationic tetramethylrhodamine methylester …

Mitochondrial polarisation status and [Ca2+] i signalling in rat cerebellar granule neurones aged in vitro

J Xiong, PJ Camello, A Verkhratsky, EC Toescu - Neurobiology of aging, 2004 - Elsevier
Mitochondrial membrane potential is a major factor that controls, ultimately, the cellular
energy supply. By use of a mitochondrial membrane potential dye (rhodamine 123, R123) …

Monitoring mitochondrial [Ca2+] dynamics with rhod-2, ratiometric pericam and aequorin

RI Fonteriz, S de la Fuente, A Moreno, CD Lobatón… - Cell calcium, 2010 - Elsevier
The dynamics of mitochondrial [Ca2+]([Ca2+] M) plays a key role in a variety of cellular
processes. The most important methods available to monitor [Ca2+] M are fluorescent dyes …