Deconstruction of lignocellulose into soluble sugars by native and designer cellulosomes

S Moraïs, E Morag, Y Barak, D Goldman, Y Hadar… - MBio, 2012 - Am Soc Microbiol
Lignocellulosic biomass, the most abundant polymer on Earth, is typically composed of three
major constituents: cellulose, hemicellulose, and lignin. The crystallinity of cellulose …

Assembly of xylanases into designer cellulosomes promotes efficient hydrolysis of the xylan component of a natural recalcitrant cellulosic substrate

S Moraïs, Y Barak, Y Hadar, DB Wilson, Y Shoham… - MBio, 2011 - Am Soc Microbiol
In nature, the complex composition and structure of the plant cell wall pose a barrier to
enzymatic degradation. Nevertheless, some anaerobic bacteria have evolved for this …

Driving biomass breakdown through engineered cellulosomes

SP Gilmore, JK Henske, MA O'Malley - Bioengineered, 2015 - Taylor & Francis
Extraction of sugar is the rate-limiting step in converting unpretreated biomass into value-
added products through microbial fermentation. Both anaerobic fungi and anaerobic …

Designer cellulosomes for enhanced hydrolysis of cellulosic substrates

Y Vazana, S Moraïs, Y Barak, R Lamed… - Methods in Enzymology, 2012 - Elsevier
During the past several years, major progress has been accomplished in the production of
“designer cellulosomes,” artificial enzymatic complexes that were demonstrated to efficiently …

The potential of cellulases and cellulosomes for cellulosic waste management

EA Bayer, R Lamed, ME Himmel - Current opinion in Biotechnology, 2007 - Elsevier
Lignocellulose is the most abundant plant cell wall component of the biosphere and the
most voluminous waste produced by our society. Fortunately, it is not toxic or directly …

Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis

J Stern, A Kahn, Y Vazana, M Shamshoum, S Morais… - PloS one, 2015 - journals.plos.org
Degradation of cellulose is of major interest in the quest for alternative sources of renewable
energy, for its positive effects on environment and ecology, and for use in advanced …

A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates

Y Vazana, Y Barak, T Unger, Y Peleg… - Biotechnology for …, 2013 - Springer
Background Select cellulolytic bacteria produce multi-enzymatic cellulosome complexes that
bind to the plant cell wall and catalyze its efficient degradation. The multi-modular …

Adaptor scaffoldins: an original strategy for extended designer cellulosomes, inspired from nature

J Stern, S Moraïs, R Lamed, EA Bayer - MBio, 2016 - Am Soc Microbiol
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled
incorporation of recombinant dockerin-containing enzymes. The largest designer …

Cellulase-xylanase synergy in designer cellulosomes for enhanced degradation of a complex cellulosic substrate

S Moraïs, Y Barak, J Caspi, Y Hadar, R Lamed… - MBio, 2010 - Am Soc Microbiol
Designer cellulosomes are precision-engineered multienzyme complexes in which the
molecular architecture and enzyme content are exquisitely controlled. This system was used …

Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability

S Moraïs, J Stern, A Kahn, AP Galanopoulou… - Biotechnology for …, 2016 - Springer
Background The concerted action of three complementary cellulases from Clostridium
thermocellum, engineered to be stable at elevated temperatures, was examined on a …