Selectable markers constitute essential genetic tools in fungal transformation. Markers find
increased application with the availability of complete genome sequences of many …

DNA-mediated transformation is a powerful tool that allows the introduction of specific
genetic changes in an organism. Transformation of Aspergilli, acclaimed for their wide use in …

To generate gene deletion mutants in Aspergillus niger, we combined the use of
nonhomologous end-joining (NHEJ) mutants (ku70 mutant) and the split marker approach …

Wild-type strains of Aspergillus niger were transformed with integrative vectors. The number
of stable transformants varied from approximately 20–30/μg up to 17,000/μg using …

Well-characterized selectable markers and promoters have been developed for the efficient
transformation of Aspergillus niger for the production of proteins and for strain construction …

Aspergillus niger is a well-known workhorse for the industrial production of enzymes and
organic acids. This fungus can also cause postharvest diseases in fruits. Although …

In this study, the efficiency of gene replacement in Aspergillus awamori between
Agrobacterium-mediated transformation and CaCl2/PEG-mediated transformation was …

CONTENTS 30.1 Introduction.................................................................................................................
............ 493 30.2 General Considerations for Gene Manipulations …

Species of the genus Aspergillus have a variety of effects on humans and have been
considered industrial cell factories due to their prominent ability for manufacturing several …

Abstract Background For Aspergillus niger, a broad set of auxotrophic and dominant
resistance markers is available. However, only few offer targeted modification of a gene of …