Our knowledge of the organization of the cell is linked, to a great extent, to light and electron
microscopy. Choosing either photons or electrons for imaging has many consequences on …

It is widely acknowledged that EM of macromolecules is entering a new phase of productivity
(for review see Nogales and Grigorieff, 2001). It is less well known that comparable …

To preserve tissue by freezing is an ancient concept going back pre sumably to the practice
of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life …

The inside of a living cell is crowded and extremely mobile at the molecular level'. For the
moment, we can only imagine, by using the theory of diffusion and our fragmentary …

The objective of modern transmission electron microscopy (TEM) in life science is to observe
biological structures in a state as close as possible to the living organism. TEM samples …

The introduction of the Balzers freeze-fracture machine by Moor in 1961 had a much greater
impact on the advancement of electron microscopy than he could have imagined. Devised …

Abstract Over the years Transmission Electron Microscopy (TEM) has evolved into a
powerful technique for the structural analysis of cells and tissues at various levels of …

Automated electron cryotomography enables the study of organelles or whole cells
embedded in vitrified ice in a close-to-life state and with a resolution of a few nanometers …

Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate
method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of …

Since the beginning of the 1980s, cryo‐electron microscopy of a thin film of vitrified aqueous
suspension has made it possible to observe biological particles in their native state, in the …