Have a Heart Obtaining pure organ preparation by microdissection from small organisms,
such as the zebrafish, Dario rerio, is itself a sufficiently arduous task. Isolating such organs in …

The zebrafish embryonic heart is composed of only a few hundred cells, representing only a
small fraction of the entire embryo. Therefore, to prevent the cardiac transcriptome from …

Zebrafish embryo becomes a popular in vivo vertebrate model for studying cardiac
development and human heart diseases due to its advantageous embryology and genetics …

Zebrafish have become a beneficial and practical model organism for the study of embryonic
heart development (see recent reviews1-6), however, work examining post-embryonic …

This protocol describes how to isolate primary cardiomyocytes from adult zebrafish hearts
and culture them for up to 4 weeks, thereby using them as an alternative to in vivo …

Traditional working heart preparations, based on the original Langendorff setup, are widely
used experimental models that have tremendously advanced the cardiovascular field …

Here we describe how to culture adult zebrafish hearts as explants and study the
regeneration of epicardial tissue ex vivo, as a means to identify therapeutic targets for heart …

Cultured cardiomyocytes can be used to study cardiomyocyte biology using techniques that
are complementary to in vivo systems. For example, the purity and accessibility of in vitro …

The zebrafish is increasingly used for cardiovascular genetic and functional studies. We
present a novel protocol to maintain and monitor whole isolated beating adult zebrafish …

We describe here a novel, fast and inexpensive method for producing a 3D 'heart'structure
that forms spontaneously, in vitro, from larval zebrafish (ZF). We have named these 3D …