Chinese hamster cells in culture were synchronized, collected at metaphase, homogenized
to release the chromosomes, and the chromosomes fractionated in a sucrose gradient using …

Isolated metaphase chromosomes from HeLa cells, mouse L cells, and Syrian hamster
embryonic fibroblast cells have been fractionated on the basis of size by linear sucrose …

The cytochemical properties of metaphase chromosomes from Chinese hamster and human
cells were studied by flow cytometry. This technique allows precise quantitation of the …

A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells
has been developed. Cells, arrested at metaphase with colchicine, were exposed to …

Isolated metaphase chromosomes of mouse L-cells, mouse L-5178Y lymphoblast tumor
cells and Chinese hamster cells have been separated into several distinct size classes by …

The metaphase chromosomes of tissue culture cells derived from four different mammalian
species have been isolated in relatively large quantities at neutral pH and essentially free of …

Publisher Summary The nucleochromatin material is extracted from interphase cells;
isolated metaphase chromosomes offer unique possibilities for studying the genetic material …

TECHNIQUES for the isolation of interphase chromosomes from nuclei of vertebrate tissues
were described in 1947 by Mirsky and Ris [19]. The determination of the chemical …

Metaphase chromosomes with high molecular weight DNA were isolated from Chinese
hamster ovary (CHO) cells in a neutral buffer containing polyamines and chelators. The …

The human karyotype can be subdivided into seven fractions containing specific
chromosomes to provide material for recombinant DNA research. The isolated metaphase …