Electron microscopy, with its ability to image DNA and nucleosomes, can provide a key
visual link in the understanding of chromatin conformation. We discuss applications of EM to …

Publisher Summary This chapter describes the cryoelectron microscopic analysis of
nucleosomes and chromatin. Cryoelectron microscopy is the technique of choice when …

In this review we have tried to cover the direct mounting techniques currently in use and,
through micrographs kindly supplied by our colleagues, to illustrate uses of the different …

Cryoelectron microscopy has been used to examine the three-dimensional (3-D)
conformation of small oligonucleosomes from chicken erythrocyte nuclei after vitrification in …

Highlights•Cryo-electro microscopy reveals the native conformation of chromatin fiber both
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …

The analytical electron microscope technique called electron spectroscopic imaging (ESI)
has a number of applications in the study of DNA: protein complexes. The method offers an …

Publisher Summary The fundamental problem in electron microscopy of chromatin concerns
specimen preparation. This chapter describes a method for visualizing soluble chromatin as …

Chromatin gels, metaphase chromosomes, and intact nuclei were studied by freeze
fracturing followed by electron microscopy. The results complement and extend those …

Publisher Summary Nuclear DNA in eukaryotes has a nucleosomal organization. Specific
assemblies of nucleosomes and nonhistone nuclear proteins play important roles in local …

Although the formation of 30-nm chromatin fibers is thought to be the most basic event of
chromatin compaction, it remains controversial because high-resolution imaging of …