A xyloglucan-specific endo-β-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme

M Pauly, LN Andersen, S Kauppinen, LV Kofod… - …, 1999 - academic.oup.com
A full-length c-DNA encoding a xyloglucan-specific endo-β-1, 4-glucanase (XEG) has been
isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast …

[HTML][HTML] Purification, characterization, cDNA cloning, and expression of a xyloglucan endoglucanase from Geotrichum sp. M128

K Yaoi, Y Mitsuishi - FEBS letters, 2004 - Elsevier
A novel xyloglucan-specific endo-β-1, 4-glucanase (XEG), xyloglucanase, with a molecular
mass of 80 kDa and a pI of 4.8, was isolated from the fungus Geotrichum sp. M128. It was …

[HTML][HTML] Functional characterization and oligomerization of a recombinant xyloglucan-specific endo-β-1, 4-glucanase (GH12) from Aspergillus niveus

ARL Damásio, LFC Ribeiro, LF Ribeiro… - … et Biophysica Acta (BBA …, 2012 - Elsevier
Xyloglucan is a major structural polysaccharide of the primary (growing) cell wall of higher
plants. It consists of a cellulosic backbone (beta-1, 4-linked glucosyl residues) that is …

Structure and expression properties of the endo-β-1,4-glucanase A gene from the filamentous fungus Aspergillus nidulans

G Chikamatsu, K Shirai, M Kato… - FEMS Microbiology …, 1999 - academic.oup.com
Abstract Endo-β-1, 4-glucanase A (EG A) of Aspergillus nidulans was purified to
homogeneity, and its genomic gene (eglA) was cloned based on partial amino acid …

Purification and Characterization of an Endo‐1,3‐β‐Glucanase from Aspergillus fumigatus

T Fontaine, RP Hartland, A Beauvais… - European journal of …, 1997 - Wiley Online Library
An endo‐1, 3‐β‐glucanase was purified from a cell wall autolysate of Aspergillus fumigatus.
This β‐glucanase activity was associated with a glycosylated 74‐kDa protein. Using a …

Cloning and Characterization of Two Xyloglucanases from Paenibacillus sp. Strain KM21

K Yaoi, T Nakai, Y Kameda, A Hiyoshi… - Applied and …, 2005 - Am Soc Microbiol
Two xyloglucan-specific endo-β-1, 4-glucanases (xyloglucanases [XEGs]), XEG5 and
XEG74, with molecular masses of 40 kDa and 105 kDa, respectively, were isolated from the …

Cloning of a gene encoding a highly stable endo-β-1, 4-glucanase from Aspergillus niger and its expression in yeast

J Hong, H Tamaki, S Akiba, K Yamamoto… - Journal of Bioscience …, 2001 - Elsevier
A gene encoding an endo-β-1, 4-glucanase, which is highly resistant to high temperature,
protease and surfactant treatment, was isolated from Aspergillus niger IFO31125 and …

[HTML][HTML] Biochemical characterization of a 27 kDa 1, 3-β-D-glucanase from Trichoderma asperellum induced by cell wall of Rhizoctonia solani

R da Silva Aires, AS Steindorff, MHS Ramada… - Carbohydrate …, 2012 - Elsevier
Trichoderma asperellum produces two extracellular 1, 3-β-d-glucanase upon induction with
cell walls from Rhizoctonia solani. A minor 1, 3-β-d-glucanase was purified to homogeneity …

Cloning, expression, and characterization of an oligoxyloglucan reducing end-specific xyloglucanobiohydrolase from Aspergillus nidulans

S Bauer, P Vasu, AJ Mort, CR Somerville - Carbohydrate research, 2005 - Elsevier
An oligoxyloglucan reducing end-specific xyloglucanobiohydrolase from the filamentous
fungus Aspergillus nidulans was cloned and expressed in Pichia pastoris as a secreted …

Cloning, expression and characterization of a family‐74 xyloglucanase from Thermobifida fusca

DC Irwin, M Cheng, B Xiang, JKC Rose… - European journal of …, 2003 - Wiley Online Library
Thermobifida fusca xyloglucan‐specific endo‐β‐1, 4‐glucanase (Xeg) 74 and the Xeg74
catalytic domain (CD) were cloned, expressed in Escherichia coli, purified and …