Mutagenesis and Crystallographic Studies of Zymomonas mobilis tRNA-Guanine Transglycosylase Reveal Aspartate 102 as the Active Site Nucleophile,

C Romier, K Reuter, D Suck, R Ficner - Biochemistry, 1996 - ACS Publications
Procaryotic tRNA-guanine transglycosylase (TGT) catalyzes the posttranscriptional base
exchange of the queuine precursor 7-aminomethyl-7-deazaguanine (preQ1) with the …

Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA‐guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity

U Grädler, R Ficner, GA Garcia, MT Stubbs… - FEBS …, 1999 - Wiley Online Library
The tRNA modifying enzyme tRNA‐guanine transglycosylase (TGT) is involved in the
exchange of guanine in the first position of the anticodon with preQ1 as part of the …

tRNA−Guanine Transglycosylase from Escherichia coli:  Molecular Mechanism and Role of Aspartate 89

JD Kittendorf, LM Barcomb, ST Nonekowski… - Biochemistry, 2001 - ACS Publications
The enzyme tRNA− guanine transglycosylase (TGT, EC 2.4. 2.29) catalyzes a
posttranscriptional transglycosylation reaction involved in the incorporation of the modified …

Crystal structure of tRNA‐guanine transglycosylase: RNA modification by base exchange.

C Romier, K Reuter, D Suck, R Ficner - The EMBO journal, 1996 - embopress.org
tRNA‐guanine transglycosylases (TGT) are enzymes involved in the modification of the
anticodon of tRNAs specific for Asn, Asp, His and Tyr, leading to the replacement of guanine …

Investigation of specificity determinants in bacterial tRNA-guanine transglycosylase reveals queuine, the substrate of its eucaryotic counterpart, as inhibitor

I Biela, N Tidten-Luksch, F Immekus, S Glinca… - PLoS …, 2013 - journals.plos.org
Bacterial tRNA-guanine transglycosylase (Tgt) catalyses the exchange of the genetically
encoded guanine at the wobble position of tRNAsHis, Tyr, Asp, Asn by the premodified base …

An essential role for aspartate 264 in catalysis by tRNA-guanine transglycosylase from Escherichia coli

JD Kittendorf, T Sgraja, K Reuter, G Klebe… - Journal of Biological …, 2003 - ASBMB
tRNA-guanine transglycosylase (TGT) catalyzes a post-transcriptional base-exchange
reaction involved in the incorporation of the modified base queuine (Q) into the wobble …

Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and …

N Tidten, B Stengl, A Heine, GA Garcia, G Klebe… - Journal of molecular …, 2007 - Elsevier
Bacterial tRNA-guanine transglycosylase (Tgt) catalyses the exchange of guanine in the
wobble position of particular tRNAs by the modified base preQ1. In vitro, however, the …

Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate

W Xie, X Liu, RH Huang - Nature Structural & Molecular Biology, 2003 - nature.com
Prokaryotic tRNA guanine transglycosylase (TGT) catalyzes replacement of guanine (G) by
7-aminomethyl-7-deazaguanine (PreQ1) at the wobble position of four specific tRNAs …

Purification, crystallization, and preliminary X‐ray diffraction studies of tRNA‐guanine transglycosylase from Zymomonas mobilis

C Romier, R Ficner, K Reuter… - … : Structure, Function, and …, 1996 - Wiley Online Library
The tRNA modifying enzyme tRNA–guanine transglycosylase (Tgt) catalyzes the exchange
of guanine in the first position of the anticodon with the queuine precursor 7‐aminomethyl‐7 …

tRNA-guanine transglycosylase from Escherichia coli: overexpression, purification and quaternary structure

GA Garcia, KA Koch, S Chong - Journal of molecular biology, 1993 - Elsevier
Abstract tRNA-guanine transglycosylase (TGT) is the enzyme responsible for the post-
transcriptional modification of specific tRNAs (Asn, Asp, His and Tyr) with queuine. In E. coli …