Detection and Quantification of Pratylenchus thornei in DNA Extracted from Soil Using Real-Time PCR

G Yan, RW Smiley, PA Okubara - Phytopathology, 2012 - Am Phytopath Society
The root-lesion nematode Pratylenchus thornei is one of the most important pests restricting
productivity of wheat in the Pacific Northwest (PNW). It is laborious and difficult to use …

Detection and Discrimination of Pratylenchus neglectus and P. thornei in DNA Extracts from Soil

G Yan, RW Smiley, PA Okubara, A Skantar… - Plant …, 2008 - Am Phytopath Society
ABSTRACT A species-specific polymerase chain reaction (PCR) method was developed to
detect and identify the root-lesion nematodes Pratylenchus neglectus and P. thornei from …

Developing a Real-Time PCR Assay for Detection and Quantification of Pratylenchus neglectus in Soil

G Yan, RW Smiley, PA Okubara, AM Skantar… - Plant …, 2013 - Am Phytopath Society
Pratylenchus neglectus is one of the most widespread and economically important
nematodes that invades plant roots and restricts wheat productivity in the Pacific Northwest …

Developing a Real-Time PCR Assay for Direct Identification and Quantification of Pratylenchus penetrans in Soil

R Baidoo, G Yan, S Nagachandrabose… - Plant …, 2017 - Am Phytopath Society
The root-lesion nematode Pratylenchus penetrans is a major pathogen of potato worldwide.
Yield losses may be exacerbated by interaction with the fungus Verticillium dahliae in the …

Specific Detection of the Root-Lesion Nematode Pratylenchus scribneri Using Conventional and Real-Time PCR

D Huang, G Yan - Plant Disease, 2017 - Am Phytopath Society
Pratylenchus scribneri is a plant-parasitic root-lesion nematode causing economic damage
to various crops worldwide. Identifying root-lesion nematodes to species using traditional …

Hydrolysis probe-based PCR for detection of Pratylenchus crenatus, P. neglectus and P. penetrans

CMG Oliveira, V Blok, R Neilson, T Mroz, D Roberts - Nematology, 2017 - brill.com
Molecular detection of pest and pathogens relies on rapid and dependable methods for their
identification as well as an assessment of their abundance. This study describes the …

Quantitative detection of the root-lesion nematode, Pratylenchus penetrans, using qPCR

F Mokrini, L Waeyenberge, N Viaene… - European Journal of …, 2013 - Springer
Pratylenchus penetrans is one of the most economically damaging plant-parasitic
nematodes and is found on a wide variety of crops. Correct identification and quantification …

Identification of Pratylenchus thornei, the cereal and legume root-lesion nematode, based on SCAR-PCR and satellite DNA

S Carrasco-Ballesteros, P Castillo, BJ Adams… - European Journal of …, 2007 - Springer
Two different molecular tools for the diagnosis of the cereal and legume root-lesion
nematode Pratylenchus thornei were developed. A randomly amplified DNA (RAPD) …

Effects of Crop Rotations and Tillage on Pratylenchus spp. in the Semiarid Pacific Northwest United States

RW Smiley, S Machado, JA Gourlie… - Plant …, 2013 - Am Phytopath Society
There is interest in converting rainfed cropping systems in the Pacific Northwest from a 2-
year rotation of winter wheat and cultivated fallow to direct-seed (no-till) systems that include …

DEVELOPMENT OF A SPECIES-SPECIFIC REVERSE PRIMER FOR THE MOLECULAR DIAGNOSTIC OF PRATYLENCHUS BRACHYURUS

ACZ Machado, LCCB Ferraz, CMG De Oliveira - Nematropica, 2007 - journals.flvc.org
Abstract Machado, ACZ, LCCB Ferraz, and CMG Oliveira. 2007. Development of a Species-
Specific Reverse Primer for the Molecular Diagnostic of Pratylenchus brachyurus …