Iron-sulfur clusters ([Fe-S] clusters) are assembled on molecular scaffolds and subsequently
used for maturation of proteins that require [Fe-S] clusters for their functions. Previous …

The nitrogen-fixing organism Azotobacter vinelandii contains at least two systems that
catalyze formation of [Fe-S] clusters. One of these systems is encoded by nif genes, whose …

The mechanism of [4Fe-4S] cluster assembly on A-type Fe–S cluster assembly proteins, in
general, and the specific role of NifIscA in the maturation of nitrogen fixation proteins are …

In recent years, it has become evident that [Fe-S] proteins, such as hydrogenase,
nitrogenase and aconitase, require a complex machinery to assemble and insert their …

An enzyme having the samel-cysteine desulfurization activity previously described for the
NifS protein was purified from a strain ofAzotobacter vinelandii deleted for the nifSgene. This …

ABSTRACT A system for the controlled expression of genes in Azotobacter vinelandii by
using genomic fusions to the sucrose catabolic regulon was developed. This system was …

The NifS and NifU proteins from Azotobacter vinelandii are required for the full activation of
nitrogenase. NifS is a homodimeric cysteine desulfurase that supplies the inorganic sulfide …

The NifU protein is a homodimer that is proposed to provide a molecular scaffold for the
assembly of [Fe-S] clusters uniquely destined for the maturation of the nitrogenase catalytic …

Abstract The NifS and NifU nitrogen fixation-specific gene products are required for the full
activation of both the Fe-protein and MoFe-protein of nitrogenase from Azotobacter …

The assembly of iron-sulfur (Fe-S) clusters, a key step in the post-translational maturation of
Fe-S proteins, is mediated by a complex apparatus. In E. coli, this process involves two …