Insufficient insulin secretion is a key component of both type 1 and type 2 diabetes. Since
insulin is released by the islets of Langerhans, obtaining viable and functional islets is …

Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood
glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional …

Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which
produces hormones for glucose regulation and other important biological functions. The …

The lack of a technique that allows mass isolation of intact, viable human islets is part of the
reason that islet transplantation has not become available to the human diabetic. This report …

SHORT ABSTRACT We describe a protocol herein for isolating intact islets from neonatal
mice. Pancreata were partially digested with collagenase, followed by washing and hand …

Despite experience, that has been obtained in the field of islet isolation in large mammals for
about the past 20 years, allo-and autotransplantation of both human and porcine islets are …

Yields and function of isolated islets vary considerably in spite of the introduction of new or
improved methods for isolation. In most studies, these variations have been attributed to …

Large yields of high-viability human islets are necessary to service the expanding
programmes of islet transplantation worldwide; similarly, there is an increasing demand from …

Background. The procedure of human islet isolation needs further optimization and
standardization. Here, we describe techniques to enhance enzymatic digestion and …

Mouse islets are commonly used in diabetes-related studies. Adequate amounts of good
quality islets are prerequisites for a reliable investigation. We describe a protocol for islet …