Publisher Summary This chapter describes the cryoelectron microscopic analysis of
nucleosomes and chromatin. Cryoelectron microscopy is the technique of choice when …

Electron microscopy, with its ability to image DNA and nucleosomes, can provide a key
visual link in the understanding of chromatin conformation. We discuss applications of EM to …

Contrast in electron cryo-microscopy (cryo-EM) is limited by the weak phase and radiation
sensitive nature of biologic samples embedded in vitrified ice. We have recently shown that …

In Eukaryotes, DNA is wound around the histone octamer forming the basic chromatin unit,
the nucleosome. Atomic structures have been obtained from crystallography and single …

Chromatin is a dynamic molecular complex composed of DNA and proteins that package the
DNA in the nucleus of eukaryotic cells. The basic structural unit of chromatin is the …

The nucleosome is the ubiquitous and fundamental DNA-protein complex of the eukaryotic
chromosome, participating in the packaging of DNA and in the regulation of gene …

We investigated the nuclear ultrastructure of HeLa cells using cryotechniques. The cells
were cultured as monolayers, quick‐frozen and cryosubstituted in acetone without chemical …

Cryoelectron microscopy has been used to examine the three-dimensional (3-D)
conformation of small oligonucleosomes from chicken erythrocyte nuclei after vitrification in …

Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of
single-particle cryo-EM in visualizing molecular structure with the ability to dissect the time …

After gradually moving away from preparation methods prone to artefacts such as plastic
embedding and negative staining for cell sections and single particles, the field of cryo …