Spontaneous changes in mitochondrial membrane potential in cultured neurons

JF Buckman, IJ Reynolds - Journal of Neuroscience, 2001 - Soc Neuroscience
Using the mitochondrial membrane potential (ΔΨm)-sensitive fluorescent dyes 5, 5′, 6, 6′-
tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) and …

A cuvette-based fluorometric analysis of mitochondrial membrane potential measured in cultured astrocyte monolayers

CJ Feeney, PS Pennefather… - Journal of neuroscience …, 2003 - Elsevier
Mitochondrial membrane potential (ΔψM) plays a key role in coordinating mitochondrial
function and cell biology in general. In astrocytes, ΔψM is an important indicator of the health …

Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria

VC Keil, F Funke, A Zeug, D Schild, M Müller - Pflügers Archiv-European …, 2011 - Springer
Using the mitochondrial potential (ΔΨ m) marker JC-1 (5, 5′, 6, 6′-tetrachloro-1, 1′, 3,
3′-tetraethylbenzimidazolylcarbocyanine iodide) and high-resolution imaging, we …

Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry

FX Sureda, E Escubedo, C Gabriel… - … : The Journal of the …, 1997 - Wiley Online Library
Mitochondrial membrane potential (MMP) in dissociated rat cerebellar neurons was
measured using rhodamine 123 (Rh 123) as fluorescent dye, and flow cytometry. Dye …

Determination of mitochondrial membrane potential and reactive oxygen species in live rat cortical neurons

DC Joshi, JC Bakowska - JoVE (Journal of Visualized Experiments), 2011 - jove.com
Mitochondrial membrane potential (ΔΨm) is critical for maintaining the physiological function
of the respiratory chain to generate ATP. A significant loss of ΔΨm renders cells depleted of …

Changes in [Ca2+]i and membrane currents during impaired mitochondrial metabolism in dissociated rat hippocampal neurons

AV Nowicky, MR Duchen - The Journal of Physiology, 1998 - Wiley Online Library
1 This study was designed to establish the basis for altered membrane excitability during the
inhibition of mitochondrial metabolism in central mammalian neurons. Perforated whole‐cell …

Mitochondrial polarisation status and [Ca2+] i signalling in rat cerebellar granule neurones aged in vitro

J Xiong, PJ Camello, A Verkhratsky, EC Toescu - Neurobiology of aging, 2004 - Elsevier
Mitochondrial membrane potential is a major factor that controls, ultimately, the cellular
energy supply. By use of a mitochondrial membrane potential dye (rhodamine 123, R123) …

Loss of mitochondrial membrane potential is associated with increase in mitochondrial volume: physiological role in neurones

D Safiulina, V Veksler, A Zharkovsky… - Journal of cellular …, 2006 - Wiley Online Library
Mitochondrial volume homeostasis is a housekeeping cellular function, thought to help
regulate oxidative capacity, apoptosis, and mechanical signaling. The volume is mainly …

Fluctuations in mitochondrial membrane potential in single isolated brain mitochondria: modulation by adenine nucleotides and Ca2+

O Vergun, IJ Reynolds - Biophysical Journal, 2004 - cell.com
In this study we investigated fluctuations in mitochondrial membrane potential (ΔΨ m) in
single isolated brain mitochondria using fluorescence imaging. Mitochondria were attached …

Mitochondrial permeability transition in the central nervous system: induction by calcium cycling‐dependent and‐independent pathways

BS Kristal, JM Dubinsky - Journal of neurochemistry, 1997 - Wiley Online Library
Isolated rat CNS mitochondria and cultured cortical astrocytes were examined for behavior
indicative of a mitochondrial permeability transition (mPT). Exposure of isolated CNS …