The potential of electron microscope tomography as a tool for obtaining three-dimensional
(3D) information about large macromolecular assemblies is greatly extended by automation …

Highlights•Cryo-electro microscopy reveals the native conformation of chromatin fiber both
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …

Non-destructive electron microscopy of native chromatin from rat liver nuclei reveals that the
30 nm fibre is formed of four 11 nm nucleofilaments, arranged in a coiled-coil (or rope-like) …

Chromatin fibers have been observed and measured in frozen hydrated sections of three
types of cell (chicken erythrocytes and sperm of Patiria miniata and Thyone briareus) …

The presence and folding pattern of chromatin in eukaryotic cells remain elusive and
controversial. In this study, we prepared ultra-thin sections of Hela cells with three different …

The three dimensional (3D) structure of chromatin fibers in sections of nuclei has been
determined using electron tomography. Low temperature embedding and nucleic acid …

Electron microscopic observations and measurements were made on thin‐sectioned
chromatin fibers and fibrils obtained from nuclei of mature chicken erythrocytes. The nuclei …

The structure of the 30‐nm chromatin fiber has provided, over the years, an important
reference in chromatin studies. Originally derived from electron microscopic studies of …

The ultrastructure of chromatin has been examined in nuclei prepared by a variety of low‐
temperature methods. Embedding glutaraldehyde (GA)‐fixed nuclei in Lowicryl K4M or …

A long strand of DNA is wrapped around the core histone and forms a nucleosome.
Although the nucleosome has long been assumed to be folded into 30-nm chromatin fibres …