A reversed-phase" high-performance" liquid chromatographic assay for total hydroxyproline
in urine is described. The urine samples are hydrolyzed overnight with acid, evaporated …

In this method for 4-hydroxyproline in urine, hydroxyproline is derivatized with 4-chloro-7-
nitrobenzofurazan, with subsequent estimation by reversed-phase" high-performance" liquid …

In this method for quantifying 4-hydroxyproline in human urine, 50 microL of urine is
hydrolyzed, derivatized with phenylisothiocyanate (PITC), and then quantified by reversed …

Three methods of determining the total hydroxyproline content in urine were compared to
check the inhibitory influence of some urinary constituents on the analysis. It is …

A simple and specific method for the determination of hydroxyproline in urine hydrolysates
has been described. Hydroxyproline was converted into its N-isobutyloxycarbonyl methyl …

A method to measure total hydroxyproline in human urine was developed. Primary amino
acids were derivatized with ortho-phthaldialdehyde, followed by derivatization of imino acids …

Phosphate-citric acid buffer (pH 5.0) comprised 0.2 M disodium monohydrogenphosphate-
O. 1 M citric acid (10.3: 9.7, v/v). Acetate-citrate-citric acid bulfer (pH 6.0) was prepared by …

Several modifications of a specific chemical assay for hydroxyproline in urine are presented
which make the procedure more rapid without reducing its specificity or accuracy. Also, a …

We report a" high-performance" liquid-chromatographic method for measuring free
hydroxyproline in serum. After separating the free hydroxyproline from the protein-and …

We have optimized a method for measuring total hydroxyproline (HYP) in urine by HPLC
after release from urinary peptides by solid-phase hydrolysis on Dowex 50W x 8 ion …