Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production

C Dong, J Qiao, X Wang, W Sun, L Chen, S Li… - Biotechnology for …, 2020 - Springer
Backgrounds Engineering yeast as a consolidated bioprocessing (CBP) microorganism by
surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol …

Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase

S Kim, SH Baek, K Lee, JS Hahn - Microbial Cell Factories, 2013 - Springer
Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but
its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this …

Functional Assembly of Minicellulosomes on the Saccharomyces cerevisiae Cell Surface for Cellulose Hydrolysis and Ethanol Production

SL Tsai, J Oh, S Singh, R Chen… - Applied and …, 2009 - Am Soc Microbiol
We demonstrated the functional display of a miniscaffoldin on the Saccharomyces
cerevisiae cell surface consisting of three divergent cohesin domains from Clostridium …

Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol

F Wen, J Sun, H Zhao - Applied and environmental microbiology, 2010 - Am Soc Microbiol
By combining cellulase production, cellulose hydrolysis, and sugar fermentation into a
single step, consolidated bioprocessing (CBP) represents a promising technology for biofuel …

In vitro assembly of minicellulosomes with two scaffoldins on the yeast cell surface for cellulose saccharification and bioethanol production

LH Fan, ZJ Zhang, XY Yu, YX Xue, MM Wang… - Process …, 2013 - Elsevier
The present paper reports in vitro strategies for assembly of minicellulosomes with two
miniscaffoldins on the Saccharomyces cerevisiae cell surface. It was carried out through …

Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome

G Goyal, SL Tsai, B Madan, NA DaSilva, W Chen - Microbial cell factories, 2011 - Springer
Background The recalcitrant nature of cellulosic materials and the high cost of enzymes
required for efficient hydrolysis are the major impeding steps to their practical usage for …

Surface display of a functional minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and …

SL Tsai, G Goyal, W Chen - Applied and Environmental …, 2010 - Am Soc Microbiol
In this paper, we report the surface assembly of a functional minicellulosome by using a
synthetic yeast consortium. The basic design of the consortium consisted of four different …

Engineering yeast with bifunctional minicellulosome and cellodextrin pathway for co-utilization of cellulose-mixed sugars

LH Fan, ZJ Zhang, S Mei, YY Lu, M Li, ZY Wang… - Biotechnology for …, 2016 - Springer
Background Consolidated bioprocessing (CBP), integrating cellulase production, cellulose
saccharification, and fermentation into one step has been widely considered as the ultimate …

Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production

LH Fan, ZJ Zhang, XY Yu, YX Xue… - Proceedings of the …, 2012 - National Acad Sciences
Yeast to directly convert cellulose and, especially, the microcrystalline cellulose into
bioethanol, was engineered through display of minicellulosomes on the cell surface of …

Constructing a yeast to express the largest cellulosome complex on the cell surface

M Anandharaj, YJ Lin, RP Rani… - Proceedings of the …, 2020 - National Acad Sciences
Cellulosomes, which are multienzyme complexes from anaerobic bacteria, are considered
nature's finest cellulolytic machinery. Thus, constructing a cellulosome in an industrial yeast …