In contrast to those of metaphase chromosomes, the shape, length, and architecture of
human interphase chromosomes are not well understood. This is mainly due to technical …

A method of in situ hybridization for visualizing individual human chromosomes from pter to
qter, both in metaphase spreads and interphase nuclei, is reported. DNA inserts from a …

Biomedical research of interphase chromosomes in their integrity is hindered by technical
limitations. We introduce a technology using microdissection-based engineering of DNA …

The study of human chromosomes in the context of an interphase nucleus is biologically
most meaningful for understanding eukaryotic DNA expression and reproduction inasmuch …

Background Chromosome banding is widely used in cytogenetics. However, the biological
nature of hierarchically organized splitting of chromosomal bands of human chromosomes is …

Human karyotype is usually studied by classical cytogenetic (banding) techniques. To
perform it, one has to obtain metaphase chromosomes of mitotic cells. This leads to the …

The three-dimensional (3D) organization of the chromosomal fiber in the human interphase
nucleus is an important but poorly understood aspect of gene regulation. Here we …

Human interphase chromosomes were simulated as a flexible fiber with excluded volume
interaction, which represents the chromatin fiber of each chromosome. For the higher-order …

Double in situ hybridization with mercurated and biotinylated chromosome specific DNA
probes in combination with digital image analysis provides a new approach to compare the …

Fluorescence in situ hybridization (FISH) of microlibraries established from distinct
chromosome subregions can test the evolutionary conservation of chromosome bands as …