Cryoelectron microscopy allows the observation of hydrated samples at high spatial
resolution, and it would be of great interest in biology to apply this method to cells and …

Cryoelectron microscopy is presently one of the most powerful methods for studying the
structure of biological material. Although there are many different preparation procedures …

Cryo-electron microscopy of vitreous sections (CEMOVIS) has proven to be a unique tool for
studying the ultrastructure of cells and tissues in their fully hydrated state (Dubochet et al …

In a recent article we have shown that thin sections of vitrified biological samples can be
prepared, transferred and observed under good conditions in the electron microscope …

Aim of this paper is to present a short overview about the state of the art of the so called
cryotechniques for electron microscopy. These protocols have been developed and …

Freezing of bulk biological objects was investigated by X‐ray cryodiffraction. Freezing at
atmospheric pressure of most microscopic biological samples gives rise to large hexagonal …

A decade after the development of electron cryo‐microscopy for vitrified specimens, its
advantages and limitations are analysed. Indeed, recent work carried out by different …

Electron microscopy of vitrified ultrathin sections allows cell ultrastructure to be studied in the
hydrated state. Sectioning of the frozen material is, however, a limiting step, since the cutting …

Our knowledge of the organization of the cell is linked, to a great extent, to light and electron
microscopy. Choosing either photons or electrons for imaging has many consequences on …

The resolution at which biological specimens can be imaged by electron microscopy is often
limited by the need to dehydrate specimens prior to their insertion in the vacuum of the …