Isolation and function of human and pig islets

S Takei, M Teruya, A Grunewald, R Garcia, EK Chan… - Pancreas, 1994 - journals.lww.com
This report confirms reproducible methods to isolate and assess viability and function of pig
and human islets. We processed 10 pig and five human pancreata. The pancreata were …

A simple method using a polymethylpenten chamber for isolation of human pancreatic islets

D Dufrane, RM Goebbels, Y Guiot, JP Squifflet… - Pancreas, 2005 - journals.lww.com
Objectives: Isolation of large numbers of intact and functional human islets remains difficult
and expensive. We describe a novel method using a polymethylpenten chamber (PMPC) …

Isolation and purification of human pancreatic islets

L Wei - Type-1 Diabetes: Methods and Protocols, 2022 - Springer
Successful islet isolation is the key to islet transplantation in diabetic patients. However, islet
isolation is a technically complex and time-consuming manual process. Optimizing the islet …

[PDF][PDF] Human pancreatic islet isolation: Part II: purification and culture of human islets

M Qi, B Barbaro, S Wang, Y Wang… - Journal of visualized …, 2009 - researchgate.net
Management of Type 1 diabetes is burdensome, both to the individual and society, costing
over 100 billion dollars annually. Despite the widespread use of glucose monitoring and …

Quantitative and qualitative standards for islet isolation assessment in humans and large mammals

C Ricordi - Pancreas, 1991 - journals.lww.com
Several procedures have been recently developed to separate and purify pancreatic islets
from large mammals, including humans. It is often difficult to interpret and compare results …

Factors influencing isolation of functional pancreatic rat islets

BJ de Haan, MM Faas, H Spijker, JW van Willigen… - Pancreas, 2004 - journals.lww.com
Yields and function of isolated islets vary considerably in spite of the introduction of new or
improved methods for isolation. In most studies, these variations have been attributed to …

Simplified method to isolate highly pure canine pancreatic islets

OO Woolcott, RN Bergman, JM Richey, EL Kirkman… - Pancreas, 2012 - journals.lww.com
Objectives The canine model has been used extensively to improve the human pancreatic
islet isolation technique. At the functional level, dog islets show high similarity to human …

Novel method for isolation of adult porcine pancreatic islets with two-stage digestion procedure

W Cui, Y Gu, M Miyamoto, M Tanaka, B Xu… - Cell …, 1999 - journals.sagepub.com
It is particularly difficult to isolate porcine islets (PI). Experience suggests that the success
rate of porcine islet isolation (PII) is probably considerably influenced by the distension and …

Improved quality and yield of islets isolated from human pancreata using a two-step digestion method

T Kenmochi, M Miyamoto, S Une, Y Nakagawa… - Pancreas, 2000 - journals.lww.com
A new approach, involving a two-step digestion process and Los Angeles preservation
solution# 1 (LAP-1), a cold storage solution, was developed for isolation of high-quality islets …

Isolation and long term preservation of pancreatic islets from mouse, rat and guinea pig

J Ferguson, RH Allsopp, RMR Taylor, IDA Johnston - Diabetologia, 1976 - Springer
This paper reports techniques for the isolation and long term preservation of pancreatic
islets from the mouse, rat and guinea pig. Islets have been isolated using a modification of a …