Novel method for isolation of adult porcine pancreatic islets with two-stage digestion procedure

W Cui, Y Gu, M Miyamoto, M Tanaka, B Xu… - Cell …, 1999 - journals.sagepub.com
It is particularly difficult to isolate porcine islets (PI). Experience suggests that the success
rate of porcine islet isolation (PII) is probably considerably influenced by the distension and …

Effective islet isolation method with extremely high islet yields from adult pigs

Y Yonekawa, S Matsumoto, T Okitsu… - Cell …, 2005 - journals.sagepub.com
Achieving good islet isolation is one of the most important factors for successful islet
transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical …

The morphology of islets within the porcine donor pancreas determines the isolation result: successful isolation of pancreatic islets can now be achieved from young …

M Krickhahn, C Bühler, T Meyer, A Thiede… - Cell …, 2002 - journals.sagepub.com
Clinical islet allotransplantation has become an increasingly efficient “routine” therapy in
recent years. Shortage of human donor organs leads to porcine pancreatic islets as a …

Improved large-scale isolation of breeder porcine islets: possibility of harvesting from nonheart-beating donor

M Miyamoto, K Inoue, Y Gu, T Tun, W Cui… - Cell …, 1998 - journals.sagepub.com
To establish a large-scale isolation procedure for adult porcine islets usable as a donor
source for xenotransplantation and as a model of human islet isolation, we improved several …

Isolation and purification of islet cells from adult pigs

AY Qiao, WH Zhang, XJ Chen, J Zhang, GH Xiao… - Transplantation …, 2010 - Elsevier
We used in situ perfusion and a multiple-organ harvesting technique to collect islets from
adult pig pancreata. The tissues were digested with collagenase P followed by purification in …

Experimental islet isolation in porcine pancreas with new enzyme Liberase PI

SC Kim, DJ Han, CH Kang, YM We, JH Back… - Transplantation …, 2004 - Elsevier
The aim of this study was to investigate the results of 20 consecutive porcine islet isolations
using a new enzyme Liberase PI. Twenty pancreata were procured for islet isolation, which …

Pancreatic islets: methods for isolation and purification of juvenile and adult pig islets

H Brandhorst, PRV Johnson, D Brandhorst - … : From the Mouse to the Clinic, 2016 - Springer
The current situation of organ transplantation is mainly determined by the disbalance
between the number of available organs and the number of patients on the waiting list. This …

Isolation of adult pig islet: In vitro assessment and xenotransplantation

K Inoue, Y Gu, S Shinohara, M Kogire, M Mitsuo… - International journal of …, 1992 - Springer
This study was conducted to develop an isolation method for adult pig islets and to
investigate in vivo function after xenotransplantation as well as in vitro function of isolated …

A modified automated method for isolation of viable pancreatic islets in laboratory animals

AO Gürol, G Yıllar, AÖ Kurşun, M Küçük, G Deniz… - Transplantation …, 2004 - Elsevier
To achieve successful islet transplantation, a high viability is required. For this reason an
automated method including two chambers: one for islets isolation and one for recirculation …

Optimizing conditions for rat pancreatic islets isolation

MM Amoli, R Moosavizadeh, B Larijani - Cytotechnology, 2005 - Springer
Many procedures have been described for rat pancreatic islet isolation. Several factors
contribute to the pancreatic islet isolation outcome. One of the main problems in islet …