Human karyotype is usually studied by classical cytogenetic (banding) techniques. To
perform it, one has to obtain metaphase chromosomes of mitotic cells. This leads to the …

The study of human chromosomes in the context of an interphase nucleus is biologically
most meaningful for understanding eukaryotic DNA expression and reproduction inasmuch …

Biomedical research of interphase chromosomes in their integrity is hindered by technical
limitations. We introduce a technology using microdissection-based engineering of DNA …

In contrast to those of metaphase chromosomes, the shape, length, and architecture of
human interphase chromosomes are not well understood. This is mainly due to technical …

Background: The concept of “chromosomics” was introduced by Prof. Uwe Claussen in
2005. Herein, the growing insights into human chromosome structure finally lead to a …

A method of in situ hybridization for visualizing individual human chromosomes from pter to
qter, both in metaphase spreads and interphase nuclei, is reported. DNA inserts from a …

After the fundamental discovery in 1956 that normal human cells contain 46 chromosomes,
clinical cytogenetics was born and studies into the relation of chromosomal defects and …

Molecular cytogenetics offers the unique possibility of investigating numerical and structural
chromosomal aberrations in interphase nuclei of somatic cells. Previous fluorescence in-situ …

Chromosome preparations currently provide the only direct view of the genome as a whole.
Although molecular methods allow a more detailed analysis of specific regions of the …

Publisher Summary This chapter describes the detection of chromosomal aberrations by
means of molecular cytogenetics. In situ hybridization is a powerful tool in oncology allowing …