Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide

JM Seo, MY Sohn, JS Suh, A Atala, JJ Yoo, YH Shon - Cryobiology, 2011 - Elsevier
Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic
applications. They can be easily mass produced, cryopreserved and shipped to clinics for …

Evaluation of distinct freezing methods and cryoprotectants for human amniotic fluid stem cells cryopreservation

FL Janz, AA Debes, RC Cavaglieri… - BioMed Research …, 2012 - Wiley Online Library
Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs)
for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing …

Safe and effective cryopreservation methods for long‐term storage of human‐amniotic‐fluid‐derived stem cells

A Hennes, L Gucciardo, S Zia, F Lesage… - Prenatal …, 2015 - Wiley Online Library
Abstract Objectives Stem cells (SCs) can be isolated from amniotic fluid (AF) for a variety of
perinatal applications. In view of this, we compared different cryopreservation protocols for …

[HTML][HTML] Efficient recovery of undifferentiated human embryonic stem cell cryopreserved with hydroxyethyl starch, dimethyl sulphoxide and serum replacement

MD Orellana, GC De Santis, KJ Abraham, AM Fontes… - Cryobiology, 2015 - Elsevier
Background The therapeutic use of human embryonic stem cells (hESCs) is dependent on
an efficient cryopreservation protocol for long-term storage. The aim of this study was to …

In situ cryopreservation of human embryonic stem cells in gas-permeable membrane culture cassettes for high post-thaw yield and good manufacturing practice

KJ Amps, M Jones, D Baker, HD Moore - Cryobiology, 2010 - Elsevier
The development of efficient and robust methods for the cryopreservation of human
embryonic stem cells (hESCs) is important for the production of master and working cell …

Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells

T Miki, W Wong, E Zhou, A Gonzalez, I Garcia… - Stem cell research & …, 2016 - Springer
Background Amnion-derived stem cells have been proposed for cell replacement therapy
and tissue regeneration. An easily accessible cell source, the placenta, allows us to …

[HTML][HTML] Human amniotic fluid stem cells (hAFSCs) expressing p21 and cyclin D1 genes retain excellent viability after freezing with (dimethyl sulfoxide) DMSO

SGG Aziz, Z Fardyazar, F Pashaei-Asl… - Bosnian Journal of …, 2019 - ncbi.nlm.nih.gov
Human amniotic fluid stem cells (hAFSCs) have features intermediate between embryonic
and adult SCs, can differentiate into lineages of all three germ layers, and do not develop …

Cryopreservation of umbilical cord blood-derived mesenchymal stem cells without dimethyl sulfoxide

HY Wang, ZR Lun, SS Lu - CryoLetters, 2011 - ingentaconnect.com
Cryopreservation of umbilical cord blood-derived mesenchymal stem cells (UCB-derived
MSCs) is crucial step for its clinical applications in cell transplantation therapy. In the …

[HTML][HTML] Effects of storage media, supplements and cryopreservation methods on quality of stem cells

OD Erol, B Pervin, ME Seker… - World Journal of Stem …, 2021 - ncbi.nlm.nih.gov
Despite a vast amount of different methods, protocols and cryoprotective agents (CPA), stem
cells are often frozen using standard protocols that have been optimized for use with cell …

Evaluation of a low cost cryopreservation system on the biology of human amniotic fluid-derived mesenchymal stromal cells

JM Miranda-Sayago, N Fernandez-Arcas, C Benito… - Cryobiology, 2012 - Elsevier
BACKGROUND: Human amniotic-derived mesenchymal stromal cells (hAMSC) are a novel
population of multipotent stem cells that have been shown to have great potential for use in …