Jeffrey C. Hansen,** Juan Ausio, ValerieH. Stanik, and KE van Holde Department of
Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331-6503 …

An oligonucleosome 12-mer was reconstituted in the absence of linker histones, onto a DNA
template consisting of 12 tandemly arranged 208-base pair fragments of the 5 S rRNA gene …

Unique roles have been identified for the histone octamer in the formation and stabilization
of higher order chromatin structures. Histone octamers were assembled onto 12 tandem …

Werstiuk, E. S „& Neilson, T.(1972) Can. J. Chem. 50, 1283-1291. of Oligonucleosomes as a
in isokinetic sucrose gradients. The results show that, de-pending on the NaCl …

We have characterized the hydrodynamic behavior of nucleosome arrays in which the N-
and C-terminal “tails” of the histone H2A-H2B and H3-H4 domains have been selectively …

Nucleosomes have been reconstituted onto dodecameric repeats of a 208-base pair
nucleosome positioning sequence at various subsaturating molar ratios of histone octamer …

Nucleosome core particles have been efficiently assembled in vitro by direct interaction of
histones and DNA at physiological ionic strength, as assayed by digestion with DNases …

Revised Manuscript Received June 10, 1991 abstract: We have examined the structures of
unique sequence, A/T-rich DNAs that are predicted to be relatively rigid [oligo (dA)* oligo …

Materials and Methods Preparation of Nucleosomal Core Particles. The 140 base pair
nucleosomal core particles were prepared by micrococcal nuclease digestion of HI depleted …

Departments of Chemistry, Biochemistry, and Biophysics and Beckman Institute, University
of Illinois, Urbana, Illinois 61801 Received May I, 1991; Revised Manuscript Received June …