Proteins excreted into the culture medium by carrot cells during somatic embryogenesis in
the absence of 2, 4-dichlorophenoxy acetic acid (2, 4-D) and during unorganized cell …

Somatic embryogenesis can be synchronized by enriching carrot (Daucus carota L.)
suspension cultures for small, dense clusters of cells termed proembryogenic masses …

The translational profile of cultured carrot cells (callus) was compared with that of the
somatic embryos derived from them. The two tissues synthesize almost the same number …

There are several biochemical differences between the callus and the embryos of carrot
culture. Callus tissue produces callus-specific proteins and a conditioning factor that is …

Embryogenic suspension cultures of domesticated carrot (Daucus carota L.) are
characterized by the presence of proembryogenic masses (PEMs) from which somatic …

Since Steward and Reinert independently discovered somatic embryogenesis in carrot
culture (Reinert, 1959; Steward et al., 1958), it has been a model system for understanding …

Nucellar-derived cell cultures of sour orange (Citrus aurantium L.) proliferate as
proembryogenic masses. By a change in the carbon source of the medium from sucrose to …

The temperature-sensitive carrot cell variant ts11c, arrested in somatic embryogenesis after
the globular stage, was characterized. The sensitivity to a shift from 24° C (permissive …

Methods Parallel cultures of haploid (HA) cells (Smith et al., 1981) were initiated at low
density (less than 4 Klett units by the turbidimetric method of Sung, 1976) into fresh B5 …

Carrot “seed” after the germination of their zygotic embryos, contain cells which can develop
in aseptic culture into somatic embros in a reliable and predictable manner without the use …