NHEJ inhibitor SCR7 and its different forms: Promising CRISPR tools for genome engineering
The CRISPR-Cas system currently stands as one of the best multifaceted tools for site-
specific genome engineering in mammals. An important aspect of research in this field …
specific genome engineering in mammals. An important aspect of research in this field …
Inhibition of nonhomologous end joining to increase the specificity of CRISPR/Cas9 genome editing
SV Vartak, SC Raghavan - The FEBS journal, 2015 - Wiley Online Library
DNA repair, one of the fundamental processes occurring in a cell, safeguards the genome
and maintains its integrity. Among various DNA lesions, double‐strand breaks are …
and maintains its integrity. Among various DNA lesions, double‐strand breaks are …
Recent advances in CRISPR‐Cas9 genome editing technology for biological and biomedical investigations
ABSTRACT The Type II CRISPR‐Cas9 system is a simple, efficient, and versatile tool for
targeted genome editing in a wide range of organisms and cell types. It continues to gain …
targeted genome editing in a wide range of organisms and cell types. It continues to gain …
[HTML][HTML] Small molecules enhance CRISPR genome editing in pluripotent stem cells
The bacterial CRISPR-Cas9 system has emerged as an effective tool for sequence-specific
gene knockout through non-homologous end joining (NHEJ), but it remains inefficient for …
gene knockout through non-homologous end joining (NHEJ), but it remains inefficient for …
Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells
Y Wang, KI Liu, NAB Sutrisnoh, H Srinivasan, J Zhang… - Genome biology, 2018 - Springer
Abstract Background While CRISPR-Cas systems hold tremendous potential for engineering
the human genome, it is unclear how well each system performs against one another in both …
the human genome, it is unclear how well each system performs against one another in both …
Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways
J Schimmel, N Munoz-Subirana, H Kool… - Cell Reports, 2023 - cell.com
Gene editing through repair of CRISPR-Cas9-induced chromosomal breaks offers a means
to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by …
to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by …
[HTML][HTML] Evolution of CRISPR towards accurate and efficient mammal genome engineering
SM Ryu, JW Hur, K Kim - BMB reports, 2019 - ncbi.nlm.nih.gov
The evolution of genome editing technology based on CRISPR (clustered regularly
interspaced short palindromic repeats) system has led to a paradigm shift in biological …
interspaced short palindromic repeats) system has led to a paradigm shift in biological …
SCR7, a potent cancer therapeutic agent and a biochemical inhibitor of nonhomologous DNA end‐joining
Background DNA double‐strand breaks (DSBs) are harmful to the cell as it could lead to
genomic instability and cell death when left unrepaired. Homologous recombination and …
genomic instability and cell death when left unrepaired. Homologous recombination and …
Approaches to enhance precise CRISPR/Cas9-mediated genome editing
Modification of the human genome has immense potential for preventing or treating disease.
Modern genome editing techniques based on CRISPR/Cas9 show great promise for altering …
Modern genome editing techniques based on CRISPR/Cas9 show great promise for altering …
To cut or not to cut: Next-generation genome editors for precision genome engineering
Since the original report of repurposing the CRISPR/Cas9 system for genome engineering,
the past decade has witnessed profound improvement in our ability to efficiently manipulate …
the past decade has witnessed profound improvement in our ability to efficiently manipulate …