Cryoelectron microscopy of vitrified sections has become a powerful tool for investigating the
fine structural features of cellular compartments. In the present study, this approach has …

Although the formation of 30-nm chromatin fibers is thought to be the most basic event of
chromatin compaction, it remains controversial because high-resolution imaging of …

Publisher Summary The chapter focuses on the application of different ultrastructural
cytochemical methods that contributed an important way to the understanding of the …

We have developed a method which allows rapid and reproducible isolation of the
outermost layer of peripheral chromatin (cortical chromatin) from interphase HeLa cell …

Highlights•Cryo-electro microscopy reveals the native conformation of chromatin fiber both
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …

We studied the chromosome periphery in human HeLa and TG cells using cryomethods in
electron microscopy. A contrasted layer of peripheral chromosomal material (PCM) was …

Publisher Summary This chapter describes the cryoelectron microscopic analysis of
nucleosomes and chromatin. Cryoelectron microscopy is the technique of choice when …

A decade after the development of electron cryo‐microscopy for vitrified specimens, its
advantages and limitations are analysed. Indeed, recent work carried out by different …

Nuclear envelope-limited chromatin sheets (ELCS) form during excessive interphase
nuclear envelope growth in a variety of cells. ELCS appear as extended sheets within the …

The technology to produce cryo-electron tomography on vitrified sections is now a few years
old and some specialised labs worldwide have gathered sufficient experience so that it is …