Abstract Over the years Transmission Electron Microscopy (TEM) has evolved into a
powerful technique for the structural analysis of cells and tissues at various levels of …

The electron microscope has made paramount contributions to understanding the structure
of biological molecules, cells, and tissues. In general, the most faithful preservation of …

During the last 70 years, transmission electron microscopy (TEM) has developed our
knowledge about ultrastructure of the cells and tissues. Another aim is the determination of …

A decade after the development of electron cryo‐microscopy for vitrified specimens, its
advantages and limitations are analysed. Indeed, recent work carried out by different …

More than 30 years ago two groups independently reported the vitrification of pure water,
which was until then regarded as impossible without a cryoprotectant [1, 2]. This opened the …

Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …

Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate
method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of …

Our knowledge of the organization of the cell is linked, to a great extent, to light and electron
microscopy. Choosing either photons or electrons for imaging has many consequences on …

Cryo-electron microscopy (cryo-EM) of unfixed, unstained, frozen-hydrated samples conveys
the most reliable view of the “live” state of cells and tissues. Advances in sample preparation …

Recently a number of new approaches have been presented with the intention to produce
electron beam transparent cryo-sections (lamellas in FIB-SEM terminology) from hydrated …