The in situ three-dimensional organization of chromatin at the nucleosome and
oligonucleosome levels is unknown. Here we use cryo-electron tomography to determine …

The compaction level of arrays of nucleosomes may be understood in terms of the balance
between the self-repulsion of DNA (principally linker DNA) and countering factors including …

The predominant view of chromatin structure is that the beaded chain of nucleosomes is
folded into a symmetrical helical fibre. Recently, however, direct evidence from cryoelectron …

The three dimensional (3D) structure of chromatin fibers in sections of nuclei has been
determined using electron tomography. Low temperature embedding and nucleic acid …

In this study, electron tomograms of plunge-frozen isolated chromatin in both open and
compacted form were recorded. We have resolved individual nucleosomes in these …

The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found
inside cells. To determine how chromatin higher-order structure is controlled, we used …

A long strand of DNA is wrapped around the core histone and forms a nucleosome.
Although the nucleosome has long been assumed to be folded into 30-nm chromatin fibres …

▪ Abstract Chromatin structure is now believed to be dynamic and intimately related with
cellular processes such as transcription. Over the past few years, high-resolution structures …

The compaction of genomic DNA into chromatin has profound implications for the regulation
of key processes such as transcription, replication and DNA repair. Nucleosomes, the …

We have previously shown that the sequential arrangement of histone-DNA contacts is
essentially the same in the nucleosomal core of sea urchin sperm nuclei, where chromatin is …