A new approach, involving a two-step digestion process and Los Angeles preservation
solution# 1 (LAP-1), a cold storage solution, was developed for isolation of high-quality islets …

The most critical factors that affect the outcome of clinical pancreatic islet transplantation are
the number and quality of donor islets available for transplantation. Toward this goal, we …

To achieve successful islet transplantation, a high viability is required. For this reason an
automated method including two chambers: one for islets isolation and one for recirculation …

Yields and function of isolated islets vary considerably in spite of the introduction of new or
improved methods for isolation. In most studies, these variations have been attributed to …

Many procedures have been described for rat pancreatic islet isolation. Several factors
contribute to the pancreatic islet isolation outcome. One of the main problems in islet …

BACKGROUND: The current technique of human pancreas digestion for islet isolation relies
on selective distribution of collagenase delivered via the pancreatic duct to produce …

The limited availability of human donors makes the search for alternative islet sources
mandatory for future developments in pancreatic islet transplantation. In this study, we report …

Objectives: Isolation of large numbers of intact and functional human islets remains difficult
and expensive. We describe a novel method using a polymethylpenten chamber (PMPC) …

The purification process is one of the most difficult procedures in pancreatic islet isolation. It
was demonstrated that the standard purification method using a COBE 2991 cell processor …

It is over a decade since the first description of cure of experimental diabetes in rats by
transplantation of isolated adult islets of Langerhans. Despite a remarkable number of …