Effective rat islet isolation is pertinent for successful islet transplantation and islet studies in
vitro. To determine which rat strain yields the highest number of pure and functional islets …

Background A major problem in the islet field is the high selectivity exercised in accepting
cadaveric pancreas for islet isolation. This practice is based on experience that indicates …

Yields and function of isolated islets vary considerably in spite of the introduction of new or
improved methods for isolation. In most studies, these variations have been attributed to …

Pancreatic islets from adult rats were cultured in artificial capillary culture units that were
perfused with nutrient medium. In this in-vitro culture system, islets remain functional for at …

Determination of the long-term function of islet transplantation in relation to the implantation
site and the numbers of islets is of scientific interest and, with human islet transplant trials in …

Isolation of islets of Langerhans from the pancreas by action of collagenase, a major
breakthrough for physiological studies in vitro, has long appeared empirical, and the results …

For clinical islet transplantation, isolated islets deteriorate rapidly in culture, although
culturing islets prior to transplantation provides flexibility for evaluation of isolated islets and …

INTRODUCTION: Human islet transplant protocols frequently incorporate a brief period of
islet culture before transplantation. The optimal medium for pretransplant islet culture is …

One of the main barriers to widespread application of islet transplantation is the limited
availability of human pancreatic islets. The reduction of graft islet mass for transplantation to …

Recent advances in islet cell transplantation have led to insulin independence in a majority
of islet transplant recipients. However, there exists a need to overcome the shortage of …