Chromatin gels, metaphase chromosomes, and intact nuclei were studied by freeze
fracturing followed by electron microscopy. The results complement and extend those …

The morphology of intact or membrane‐deprived interphase nuclei has been analysed by
freeze‐fracture electron microscopy. This method appears particularly useful for providing …

The ultrastructure of chromatin has been examined in nuclei prepared by a variety of low‐
temperature methods. Embedding glutaraldehyde (GA)‐fixed nuclei in Lowicryl K4M or …

Interphase rat liver nuclei were studied by freeze fracturing followed by electron microscopic
observations. This method permits information on the native organization of the nuclear …

Publisher Summary This chapter discusses some methods for preparation of chromatin and
chromosomes for electron microscopy. Analysis of chromatin structure in situ must be done …

The freeze‐fracture thaw‐fix (FfTF) technique described in earlier papers is applied in the
present work to more detailed study of the chicken erythrocyte, by transmission replicas and …

Electron microscopy, with its ability to image DNA and nucleosomes, can provide a key
visual link in the understanding of chromatin conformation. We discuss applications of EM to …

Glutaraldehyde and formaldehyde have been used to fix chromatin core particles for
electron microscopy. Glutaraldehyde crosslinks protein only, whereas formaldehyde …

We describe a modification of the spreading method of Miller and Bakken [17]. Prior to
mounting the samples, the carbon supports used for electron microscopy are coated with …

A variety of studies (see, eg, this volume) have shown that chromatin consists of nuclease-
resistant subunits (nucleosomes). Electron micrographs of chromatin, formaldehyde-fixed …