The predominant view of chromatin structure is that the beaded chain of nucleosomes is
folded into a symmetrical helical fibre. Recently, however, direct evidence from cryoelectron …

The three dimensional (3D) structure of chromatin fibers in sections of nuclei has been
determined using electron tomography. Low temperature embedding and nucleic acid …

The" 30-nm" chromatin fibers, as observed in eukaryotic nuclei, are considered a discrete
level in a hierarchy of DNA folding. At present, there is considerable debate as to how the …

A long strand of DNA is wrapped around the core histone and forms a nucleosome.
Although the nucleosome has long been assumed to be folded into 30-nm chromatin fibres …

The question as to how the strands of chromatin are packed into the eukaryotic nucleus has
intrigued biologists and biochemists for many years. Although there is ample space in the …

We show that the mechanism by which chromatin displaying higher-order structure is
usually isolated from nuclei involves a transition to an extended nucleosomal arrangement …

The last years have witnessed rapid progress in our understanding of chromatin structure at
the nucleosome or beads-on-a-string level (Elgin and Weintraub 1975; Kornberg 1977) …

The in situ three-dimensional organization of chromatin at the nucleosome and
oligonucleosome levels is unknown. Here we use cryo-electron tomography to determine …

Highlights•Cryo-electro microscopy reveals the native conformation of chromatin fiber both
in vitro and in situ.•Presence of linker histone and ionic conditions determines the level of …

The linear array of nucleosomes that comprises the primary structure of chromatin is folded
and condensed to varying degrees in nuclei and chromosomes forming 'higher order …