Yields and function of isolated islets vary considerably in spite of the introduction of new or
improved methods for isolation. In most studies, these variations have been attributed to …

A new approach, involving a two-step digestion process and Los Angeles preservation
solution# 1 (LAP-1), a cold storage solution, was developed for isolation of high-quality islets …

This report confirms reproducible methods to isolate and assess viability and function of pig
and human islets. We processed 10 pig and five human pancreata. The pancreata were …

Many procedures have been described for rat pancreatic islet isolation. Several factors
contribute to the pancreatic islet isolation outcome. One of the main problems in islet …

There have been no extensive studies investigating the effect of tissue culture media on the
in vitro functional characteristics of rat, porcine and human Islets of Langerhans. We …

It is particularly difficult to isolate porcine islets (PI). Experience suggests that the success
rate of porcine islet isolation (PII) is probably considerably influenced by the distension and …

A modified collagenase digestion method is described for the isolation of large numbers of
islets from the canine pancreas (∼ 3500 islets/g). The islets isolated by this technique …

Various conditions for tissue culture of collagenase-isolated mouse pancreatic islets were
studied in an attempt to optimize the maintenance of glucose stimulated insulin biosynthesis …

Effective rat islet isolation is pertinent for successful islet transplantation and islet studies in
vitro. To determine which rat strain yields the highest number of pure and functional islets …

Insufficient insulin secretion is a key component of both type 1 and type 2 diabetes. Since
insulin is released by the islets of Langerhans, obtaining viable and functional islets is …