Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of
single-particle cryo-EM in visualizing molecular structure with the ability to dissect the time …

Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades
as a technique capable of studying challenging systems that otherwise defy structural …

Background Since the introduction of what became today's standard for cryo-embedding of
biological macromolecules at native conditions more than 30 years ago, techniques and …

The suddenness with which single-particle cryo-electron microscopy (cryo-EM) has
emerged as a method for determining high-resolution structures of biological …

With the advent of direct electron detectors, cryo-EM has become a popular choice for
molecular structure determination. Among its advantages over X-ray crystallography are (1) …

Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique
that has experienced major advances in recent years. Technological developments in image …

Light and electron cryo-microscopy have each proven to be powerful tools to study
biological structures in a near-native state. Light microscopy provides important localization …

There has been enormous progress during the last few years in the determination of three-
dimensional biological structures by single particle electron cryomicroscopy (cryoEM) …

Macromolecular machines carry out many cellular functions. Cryo-electron microscopy (cryo-
EM) is emerging as a powerful method for studying the structure, assembly and dynamics of …

▪ Abstract Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (ie,
unordered, nonaggregated) form is a new approach to the study of molecular assemblies …