Enzymes of hydrogen metabolism in Pyrococcus furiosus

PJ Silva, ECD van den Ban, H Wassink… - European Journal of …, 2000 - Wiley Online Library
The genome of Pyrococcus furiosus contains the putative mbhABCDEFGHIJKLMN operon
for a 14‐subunit transmembrane complex associated with a Ni–Fe hydrogenase. Ten ORFs …

Further characterization of the [Fe]‐hydrogenase from Desulfovibrio desulfuricans ATCC 7757

EC Hatchikian, N Forget, VM Fernandez… - European journal of …, 1992 - Wiley Online Library
The properties of the periplasmic hydrogenase from Desulfovibrio desulfuricans ATCC
7757, previously reported to be a single‐subunit protein [Glick, BR, Martin, WG and Martin …

Carboxy-Terminal Processing of the Large Subunit of [Fe] Hydrogenase from Desulfovibrio desulfuricans ATCC 7757

EC Hatchikian, V Magro, N Forget, Y Nicolet… - Journal of …, 1999 - Am Soc Microbiol
ABSTRACT hydA and hydB, the genes encoding the large (46-kDa) and small (13.5-kDa)
subunits of the periplasmic [Fe] hydrogenase from Desulfovibrio desulfuricans ATCC 7757 …

Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic ArchaeonPyrococcus furiosus

R Sapra, MFJM Verhagen, MWW Adams - Journal of bacteriology, 2000 - Am Soc Microbiol
Highly washed membrane preparations from cells of the hyperthermophilic archaeon
Pyrococcus furiosus contain high hydrogenase activity (9.4 μmol of H2 evolved/mg at 80° C) …

Reinvestigation of the Steady-State Kinetics and Physiological Function of the Soluble NiFe-Hydrogenase I of Pyrococcus furiosus

DJ van Haaster, PJ Silva, PL Hagedoorn… - Journal of …, 2008 - Am Soc Microbiol
Pyrococcus furiosus has two types of NiFe-hydrogenases: a heterotetrameric soluble
hydrogenase and a multimeric transmembrane hydrogenase. Originally, the soluble …

Homologous Expression of a Subcomplex of Pyrococcus furiosus Hydrogenase that Interacts with Pyruvate Ferredoxin Oxidoreductase

RC Hopkins, J Sun, FE Jenney Jr, SK Chandrayan… - PloS one, 2011 - journals.plos.org
Hydrogen gas is an attractive alternative fuel as it is carbon neutral and has higher energy
content per unit mass than fossil fuels. The biological enzyme responsible for utilizing …

Molecular Study and Partial Characterization of Iron-only Hydrogenase inDesulfovibrio fructosovorans

L Casalot, CE Hatchikian, N Forget, P de Philip… - Anaerobe, 1998 - Elsevier
An iron-only hydrogenase was partially purified and characterized fromDesulfovibrio
fructosovoranswild-type strain. The enzyme exhibits a molecular mass of 56 kDa and is …

Intact functional fourteen-subunit respiratory membrane-bound [NiFe]-hydrogenase complex of the hyperthermophilic archaeon Pyrococcus furiosus

PM McTernan, SK Chandrayan, CH Wu… - Journal of Biological …, 2014 - ASBMB
The archaeon Pyrococcus furiosus grows optimally at 100° C by converting carbohydrates to
acetate, CO 2, and H 2, obtaining energy from a respiratory membrane-bound hydrogenase …

Nucleotide sequence of the gene encoding the hydrogenase from Desulfovibrio vulgaris (Hildenborough)

G Voordouw, S Brenner - European Journal of Biochemistry, 1985 - Wiley Online Library
The nucleotide sequence of the 4.7‐kb Sa/I/EcoRI insert of plasmid pHV15 containing the
hydrogenase gene from Desulfovibrio vulgaris (Hildenborough) has been determined with …

Engineering hyperthermophilic archaeon Pyrococcus furiosus to overproduce its cytoplasmic [NiFe]-hydrogenase

SK Chandrayan, PM McTernan, RC Hopkins… - Journal of Biological …, 2012 - ASBMB
The cytoplasmic hydrogenase (SHI) of the hyperthermophilic archaeon Pyrococcus furiosus
is an NADP (H)-dependent heterotetrameric enzyme that contains a nickel-iron catalytic site …