Multiphoton imaging of freezing and heating effects in plant leaves

HG Breunig, F Tümer, K König - Journal of Biophotonics, 2013 - Wiley Online Library
Thermally‐induced changes in Arabidopsis thaliana leaves were investigated with a novel
cryo microscope by multiphoton, fluorescence lifetime and spectral imaging as well as micro …

Multiphoton imaging of biological samples during freezing and heating

HG Breunig, A Uchugonova… - Multiphoton Microscopy in …, 2014 - spiedigitallibrary.org
We applied multiphoton microscopic imaging to observe freezing and heating effects in plant-
and animal cell samples. The experimental setups consisted of a multiphoton imaging …

Multiphoton cryo microscope with sample temperature control

HG Breunig, A Uchugonova… - Multiphoton Microscopy in …, 2013 - spiedigitallibrary.org
We present a multiphoton microscope system which combines the advantages of
multiphoton imaging with precise control of the sample temperature. The microscope …

Two-photon imaging of intact living plants during freezing with a flexible multiphoton tomograph

HG Breunig, K König - Laser Physics Letters, 2015 - iopscience.iop.org
We describe the combination of a flexible multiphoton tomograph (MPTflex) with a heating
and cooling stage. The stage allows temperature control in the range of (− 196 C)(77 K) to+ …

Design and construction of a new temperature‐controlled chamber for light and confocal microscopy under monitored conditions: biological application for plant …

O Buchner, C Lütz, A Holzinger - Journal of Microscopy, 2007 - Wiley Online Library
A new light microscope–temperature‐controlled chamber (LM–TCC) has been constructed.
The special feature of the light microscope–temperature‐controlled chamber is the Peltier …

Promising monitoring techniques for plant science: Thermal and chlorophyll fluorescence imaging

A Saglam, L Chaerle… - Photosynthesis …, 2019 - Wiley Online Library
Plant physiological parameters can be assessed in vivo without contact and in a non‐
destructive way using imaging techniques. The discriminating power of thermal and …

[HTML][HTML] Infrared and chlorophyll fluorescence imaging methods for stress evaluation

DM Glenn - HortScience, 2012 - journals.ashs.org
Infrared and chlorophyll fluorescence imaging methods are useful techniques to evaluate
environmental effects on plant performance. With the advent of digital imaging and …

Chlorophyll thermofluorescence and thermoluminescence as complementary tools for the study of temperature stress in plants

JM Ducruet, V Peeva, M Havaux - Photosynthesis Research, 2007 - Springer
The photosynthetic apparatus, especially the electron transport chain imbedded in the
thylakoid membrane, is one of the main targets of cold and heat stress in plants. Prompt and …

Thermoimaging as a tool for studying light-induced heating of leaves: Correlation of heat dissipation with the efficiency of photosystem II photochemistry and non …

R Kaňa, I Vass - Environmental and Experimental Botany, 2008 - Elsevier
Thermoimaging–a highly sensitive and non-invasive method of temperature measurement–
was applied to explore the role of changing photosynthetic efficiency in light-induced …

Picosecond time-gated microscopy of UV-damaged plant tissue

S Rehman, PB Lukins - Optics Express, 2002 - opg.optica.org
We demonstrate that picosecond time-gated fluorescence microscopy can be used to
monitor subtle changes in the kinetics and spatial distribution of perturbations to the …