[HTML][HTML] The technology and biology of single-cell RNA sequencing

AA Kolodziejczyk, JK Kim, V Svensson, JC Marioni… - Molecular cell, 2015 - cell.com
The differences between individual cells can have profound functional consequences, in
both unicellular and multicellular organisms. Recently developed single-cell mRNA …

An introduction to the analysis of single-cell RNA-sequencing data

AA AlJanahi, M Danielsen, CE Dunbar - Molecular Therapy Methods & …, 2018 - cell.com
The recent development of single-cell RNA sequencing has deepened our understanding of
the cell as a functional unit, providing new insights based on gene expression profiles of …

Single-cell RNA-sequencing: the future of genome biology is now

S Picelli - RNA biology, 2017 - Taylor & Francis
Genome-wide single-cell analysis represents the ultimate frontier of genomics research. In
particular, single-cell RNA-sequencing (scRNA-seq) studies have been boosted in the last …

[HTML][HTML] Single cells make big data: new challenges and opportunities in transcriptomics

P Angerer, L Simon, S Tritschler, FA Wolf… - Current opinion in …, 2017 - Elsevier
Recent technological advances have enabled unprecedented insight into transcriptomics at
the level of single cells. Single cell transcriptomics enables the measurement of …

[HTML][HTML] Single-cell transcriptome sequencing: recent advances and remaining challenges

S Liu, C Trapnell - F1000Research, 2016 - ncbi.nlm.nih.gov
Single-cell RNA-sequencing methods are now robust and economically practical and are
becoming a powerful tool for high-throughput, high-resolution transcriptomic analysis of cell …

Single‐cell sequencing methodologies: from transcriptome to multi‐dimensional measurement

Y Chen, J Song, Q Ruan, X Zeng, L Wu, L Cai… - Small …, 2021 - Wiley Online Library
Cells are the basic building blocks of biological systems, with inherent unique molecular
features and development trajectories. The study of single cells facilitates in‐depth …

Entering the era of single-cell transcriptomics in biology and medicine

R Sandberg - Nature methods, 2014 - nature.com
Recent technical advances have enabled RNA sequencing (RNA-seq) in single cells.
Exploratory studies have already led to insights into the dynamics of differentiation, cellular …

Single-cell RNA sequencing: technical advancements and biological applications

E Hedlund, Q Deng - Molecular aspects of medicine, 2018 - Elsevier
Cells are the basic building blocks of organisms and each cell is unique. Single-cell RNA
sequencing has emerged as an indispensable tool to dissect the cellular heterogeneity and …

RNA-sequencing from single nuclei

RV Grindberg, JL Yee-Greenbaum… - Proceedings of the …, 2013 - National Acad Sciences
It has recently been established that synthesis of double-stranded cDNA can be done from a
single cell for use in DNA sequencing. Global gene expression can be quantified from the …

Quantitative assessment of single-cell RNA-sequencing methods

AR Wu, NF Neff, T Kalisky, P Dalerba, B Treutlein… - Nature …, 2014 - nature.com
Interest in single-cell whole-transcriptome analysis is growing rapidly, especially for profiling
rare or heterogeneous populations of cells. We compared commercially available single-cell …