Seit den 1970ger Jahren sind auf der Oberfläche von Zellen so gut wie aller Gewebe
Ektoproteinkinasen nachgewiesen worden. Die bis heute bekannten Substrate dieser …

In an attempt to elucidate the intracellular events regulating the proliferation of endothelial
cells (EC), we have compared the phosphorylation events in membranes prapared from …

A rapid approach for detecting tentative membrane proteins which are transiently
phosphorylated/dephosphorylated is described. Cell fractionation is unnecessary, as are …

Extracellular protein kinase activity is demonstrated in intact cultured porcine aortic
endothelial cells and is characterised. When cells were incubated with [γ-32 P] ATP (1 μM) a …

Little is known of the intracellular events which regulate the proliferation of endothelial cells
(EC). Triton-solubilized membranes from proliferating (sparse) and quiescent (confluent) EC …

The activation of various kinase systems during the proliferative response of fibroblasts and
certain tumor cell lines is well documented. The intracellular signalling pathway involved in …

A phenol-acetic acid-urea polyacrylamide gel electrophoretic system (PAU-PAGE) was
simplified by adaptation to a slab gel format, allowing the simultaneous comparison of up to …

Membrane proteases that are detectable by cytochemical means are the classified
exopeptidases, aminopeptidases A and M (or N), γ-glutamyl transpeptidase (which also acts …

The 1iA. hy 926 cell line is a permanent human endothelial cell line originating from the
fusion of primary human umbilical vein endothelial cells and cells from the human lung …

This chapter describes a procedure for isolation and analysis of fractions enriched in plasma
membranes from minute amounts of tissue. It consists of a method for extraction and …