Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing

SST Mak, S Gopalakrishnan, C Carøe, C Geng… - …, 2017 - academic.oup.com
Ancient DNA research has been revolutionized following development of next-generation
sequencing platforms. Although a number of such platforms have been applied to ancient …

Improving ancient DNA read mapping against modern reference genomes

M Schubert, A Ginolhac, S Lindgreen, JF Thompson… - BMC genomics, 2012 - Springer
Abstract Background Next-Generation Sequencing has revolutionized our approach to
ancient DNA (aDNA) research, by providing complete genomic sequences of ancient …

True single-molecule DNA sequencing of a pleistocene horse bone

L Orlando, A Ginolhac, M Raghavan, J Vilstrup… - Genome …, 2011 - genome.cshlp.org
Second-generation sequencing platforms have revolutionized the field of ancient DNA,
opening access to complete genomes of past individuals and extinct species. However …

Comparing the performance of three ancient DNA extraction methods for high‐throughput sequencing

C Gamba, K Hanghøj, C Gaunitz… - Molecular Ecology …, 2016 - Wiley Online Library
The DNA molecules that can be extracted from archaeological and palaeontological
remains are often degraded and massively contaminated with environmental microbial …

Characterization of ancient and modern genomes by SNP detection and phylogenomic and metagenomic analysis using PALEOMIX

M Schubert, L Ermini, CD Sarkissian, H Jónsson… - Nature protocols, 2014 - nature.com
Next-generation sequencing technologies have revolutionized the field of paleogenomics,
allowing the reconstruction of complete ancient genomes and their comparison with modern …

A reference human genome dataset of the BGISEQ-500 sequencer

J Huang, X Liang, Y Xuan, C Geng, Y Li, H Lu… - …, 2017 - academic.oup.com
Background: BGISEQ-500 is a new desktop sequencer developed by BGI. Using DNA
nanoball and combinational probe anchor synthesis developed from Complete Genomics™ …

Library construction for ancient genomics: single strand or double strand?

EA Bennett, D Massilani, G Lizzo, J Daligault… - …, 2014 - Taylor & Francis
A novel method of library construction that takes advantage of a single-stranded DNA ligase
has been recently described and used to generate high-resolution genomes from ancient …

Single‐tube library preparation for degraded DNA

C Carøe, S Gopalakrishnan, L Vinner… - Methods in Ecology …, 2018 - Wiley Online Library
In recent years, massive parallel sequencing has revolutionised the study of degraded DNA,
thus enabling the field of ancient DNA to evolve into that of paleogenomics. Despite these …

Experimental conditions improving in‐solution target enrichment for ancient DNA

DI Cruz‐Dávalos, B Llamas, C Gaunitz… - Molecular ecology …, 2017 - Wiley Online Library
High‐throughput sequencing has dramatically fostered ancient DNA research in recent
years. Shotgun sequencing, however, does not necessarily appear as the best‐suited …

From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing

M Meyer, AW Briggs, T Maricic, B Höber… - Nucleic Acids …, 2008 - academic.oup.com
Current efforts to recover the Neandertal and mammoth genomes by 454 DNA sequencing
demonstrate the sensitivity of this technology. However, routine 454 sequencing …