Toward characterizing extracellular vesicles at a single-particle level

C Chiang, C Chen - Journal of biomedical science, 2019 - Springer
Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles that serve a means
of cell-cell communication. Studying EVs at a single-particle level is important because EVs …

Analytical challenges of extracellular vesicle detection: A comparison of different techniques

U Erdbrügger, J Lannigan - Cytometry Part A, 2016 - Wiley Online Library
The interest in extracellular vesicles (EVs) has grown exponentially over the last decade.
Evolving evidence is demonstrating that these EVs are playing an important role in health …

Extracellular vesicle quantification and characterization: common methods and emerging approaches

TA Hartjes, S Mytnyk, GW Jenster, V van Steijn… - Bioengineering, 2019 - mdpi.com
Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information
about cells by which they are secreted. Growing interest in the role of EVs in intercellular …

Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles

G Bordanaba-Florit, F Royo, SG Kruglik… - Nature …, 2021 - nature.com
Extracellular vesicles (EVs) are heterogeneous lipid containers with a complex molecular
cargo comprising several populations with unique roles in biological processes. These …

Overview of extracellular vesicle characterization techniques and introduction to combined reflectance and fluorescence confocal microscopy to distinguish …

C Bağcı, M Sever-Bahcekapili, N Belder… - …, 2022 - spiedigitallibrary.org
Extracellular vesicles (EVs) are nanoparticles (30 to 1000 nm in diameter) surrounded by a
lipid-bilayer which carry bioactive molecules between local and distal cells and participate in …

Methods to analyze EVs

B Giebel, C Helmbrecht - Exosomes and Microvesicles: Methods and …, 2017 - Springer
Research in the field of extracellular vesicles (EVs) is challenged by the small size of the
nano-sized particles. Apart from the use of transmission and scanning electron microscopy …

Opportunities and pitfalls of fluorescent labeling methodologies for extracellular vesicle profiling on high-resolution single-particle platforms

D Fortunato, D Mladenović, M Criscuoli, F Loria… - International Journal of …, 2021 - mdpi.com
The relevance of extracellular vesicles (EVs) has grown exponentially, together with
innovative basic research branches that feed medical and bioengineering applications …

Methods to analyze extracellular vesicles at single particle level

Y Kwon, J Park - Micro and Nano Systems Letters, 2022 - Springer
Extracellular vesicles (EVs) are nano-sized vesicles derived from cells that transport
biomaterials between cells through biofluids. Due to their biological role and components …

Analysis of individual extracellular vesicles by imaging flow cytometry

T Tertel, A Görgens, B Giebel - Methods in enzymology, 2020 - Elsevier
Virtually all cells release extracellular vesicles (EVs) into their environment, such as
exosomes and microvesicles. EVs can mediate intercellular communication processes in a …

Alternative methods for characterization of extracellular vesicles

F Momen-Heravi, L Balaj, S Alian, J Tigges… - Frontiers in …, 2012 - frontiersin.org
Extracellular vesicles (ECVs) are nano-sized vesicles released by all cells in vitro as well as
in vivo. Their role has been implicated mainly in cell–cell communication, but also in disease …