A replication-competent vesicular stomatitis virus for studies of SARS-CoV-2 spike-mediated cell entry and its inhibition

ME Dieterle, D Haslwanter, RH Bortz… - Cell host & …, 2020 - cell.com
There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid
SARS-CoV-2 countermeasure development is contingent on the availability of robust …

Neutralizing activity of Sputnik V vaccine sera against SARS-CoV-2 variants

S Ikegame, MNA Siddiquey, CT Hung, G Haas… - Nature …, 2021 - nature.com
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected at least 180
million people since its identification as the cause of the current COVID-19 pandemic. The …

Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells

HL Xiong, YT Wu, JL Cao, R Yang, YX Liu… - Emerging microbes & …, 2020 - Taylor & Francis
The global pandemic of coronavirus disease 2019 (COVID-19) is a disaster for human
society. A convenient and reliable neutralization assay is very important for the development …

Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein

H Tani, M Kimura, L Tan, Y Yoshida, T Ozawa, H Kishi… - Virology journal, 2021 - Springer
Background SARS-CoV-2 is a novel coronavirus that emerged in 2019 and is now classified
in the genus Coronavirus with closely related SARS-CoV. SARS-CoV-2 is highly pathogenic …

Protocol and reagents for pseudotyping lentiviral particles with SARS-CoV-2 spike protein for neutralization assays

KHD Crawford, R Eguia, AS Dingens, AN Loes… - Viruses, 2020 - mdpi.com
SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing
antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated …

[HTML][HTML] Replication-competent vesicular stomatitis virus vaccine vector protects against SARS-CoV-2-mediated pathogenesis in mice

JB Case, PW Rothlauf, RE Chen, NM Kafai, JM Fox… - Cell host & …, 2020 - cell.com
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused millions of
human infections, and an effective vaccine is critical to mitigate coronavirus-induced disease …

Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses

F Schmidt, Y Weisblum, F Muecksch… - Journal of Experimental …, 2020 - rupress.org
The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID-19 disease
has generated a need for assays to rapidly and conveniently measure the antiviral activity of …

Generation of SARS-CoV-2 spike pseudotyped virus for viral entry and neutralization assays: a 1-week protocol

JM Condor Capcha, G Lambert… - Frontiers in …, 2021 - frontiersin.org
The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus requires reliable assays
for studying viral entry mechanisms which remains poorly understood. This knowledge is …

Optimized pseudotyping conditions for the SARS-COV-2 spike glycoprotein

MC Johnson, TD Lyddon, R Suarez, B Salcedo… - Journal of …, 2020 - Am Soc Microbiol
The severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) Spike glycoprotein is
solely responsible for binding to the host cell receptor and facilitating fusion between the …

Rapid quantification of SARS-CoV-2-neutralizing antibodies using propagation-defective vesicular stomatitis virus pseudotypes

F Zettl, TL Meister, T Vollmer, B Fischer, J Steinmann… - Vaccines, 2020 - mdpi.com
Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2, a new member of the
genus Betacoronavirus, is a pandemic virus, which has caused numerous fatalities …